Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin.

Applied and Environmental Microbiology
Rongzhi WangWenxiong Lin

Abstract

Vibrio parahaemolyticus is a halophilic bacterium that is widely distributed in water resources. The bacterium causes lethal food-borne diseases and poses a serious threat to human and animal health all over the world. The major pathogenic factor of V. parahaemolyticus is thermolabile hemolysin (TLH), encoded by the tlh gene, but its toxicity mechanisms are unknown. A high-affinity antibody that can neutralize TLH activity effectively is not available. In this study, we successfully expressed and purified the TLH antigen and discovered a high-affinity antibody to TLH, named scFv-LA3, by phage display screening. Cytotoxicity analysis showed that scFv-LA3 has strong neutralization effects on TLH-induced cell toxicity.

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Citations

Oct 17, 2014·Indian Journal of Microbiology·Rongzhi WangShihua Wang
Mar 13, 2014·Toxicon : Official Journal of the International Society on Toxinology·Rongzhi WangShihua Wang
Sep 13, 2014·Biotechnology Letters·Bee Nar LimTheam Soon Lim
Mar 24, 2015·Frontiers in Microbiology·Rongzhi WangShihua Wang
Sep 14, 2016·Journal of Agricultural and Food Chemistry·Rongzhi WangShihua Wang
May 21, 2016·Proteomics. Clinical Applications·Philipp KuhnMichael Hust
May 6, 2014·Applied and Environmental Microbiology·Rongzhi WangShihua Wang
Apr 13, 2017·Frontiers in Microbiology·Abdullah F U H SaeedShihua Wang
Mar 23, 2018·Frontiers in Microbiology·Hang YangRongzhi Wang
Jul 7, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Yaoguang ChenNing Shi
Apr 15, 2020·Journal of Microbiological Methods·Taro YonekitaTakashi Matsumoto
Jul 27, 2021·Frontiers in Cellular and Infection Microbiology·Kristian Daniel Ralph RothMichael Hust

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