Screening for anabolic steroids in sports: analytical strategy based on the detection of phase I and phase II intact urinary metabolites by liquid chromatography tandem mass spectrometry

Journal of Chromatography. a
Georgina BalcellsRosa Ventura

Abstract

In order to improve the detection capabilities of anabolic androgenic steroids (AAS) in sports, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for the simultaneous detection of AAS phase I and phase II intact urinary metabolites (glucuronides and sulfates) was developed. A total of 36 metabolites (7 unconjugated; 19 glucuronides and 10 sulfates) corresponding to 15 of the most reported AAS were included. Analytes were extracted from urine using C18 cartridges. LC and MS conditions were studied in-depth to determine the most sensitive and selective conditions for each analyte. A selected reaction monitoring method was set up. The optimization of the experimental parameters for 13 metabolites not available as standards was performed using excretion study urines. Extraction recoveries were above 77% for all 23 validated analytes. Intra-day precision was lower than 21%, and LODs were in the range 0.25-4ng/mL for 18 of the 23 analytes. Matrix effect was evaluated using post column infusion and ranged from 92 to 147%. The method was successfully applied to excretion study urines of different exogenous AAS. The suitability of the strategy was demonstrated with methyltestosterone and stanozolol excretion s...Continue Reading

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Citations

Jan 16, 2016·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Dec 29, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Josep Marcos, Oscar J Pozo
Apr 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Georgina BalcellsRosa Ventura
May 31, 2016·Drug Testing and Analysis·Argitxu EsquivelRosa Ventura
Jul 10, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S Rzeppa, L Viet
Aug 25, 2016·Rapid Communications in Mass Spectrometry : RCM·Yuanyuan YaoRoger W Giese
Jul 22, 2017·Journal of Mass Spectrometry : JMS·Argitxu EsquivelRosa Ventura
May 16, 2020·Bioanalysis·Biljana Jancic StojanovicGmeiner Günter
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Steffen LokeMaria Kristina Parr

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