Screening for differential methylation status in human placenta in preeclampsia using a CpG island plus promoter microarray

International Journal of Molecular Medicine
Rui-Zhe JiaHong-Juan Ding

Abstract

The development of preeclampsia (PE) seriously affects the health of the mother and the child, but the precise pathogenesis of PE remains elusive. The placenta is considered to play a key role and DNA methylation may be associated with altered placental development and function. The aim of this study was to perform a genome-wide analysis of the DNA methylation profile in placentas from pregnancies with severe preeclampsia. The authors analyzed normal and placental tissues with PE for aberrant DNA methylation using methylated DNA immunoprecipitation (MeDIP) and a human CpG island plus promoter microarray. The methylation status of identified candidate genes were validated by bisulfite sequencing PCR (BSP). Microarray analysis identified 296 genes that showed significantly aberrant DNA methylation in preeclampsia (PE). These genes were located more frequently in chromosome 1 (10.5%, P=0.005), chromosome 12 (8.1%, P=0.062) and chromosome 19 (7.4%, P=0.117). Functional analysis divided these genes into different functional networks. In addition, the methylation profile of six of these genes (CAPN2, EPHX2, ADORA2B, SOX7, CXCL1 and CDX1) in nine patients with PE was validated by BSP. This study demonstrated aberrant patterns of DNA m...Continue Reading

Citations

May 20, 2014·Acta Obstetricia Et Gynecologica Scandinavica·Richard Saffery, Boris Novakovic
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Vania JanuarRichard Saffery
Sep 12, 2015·Nutrition Reviews·Mansi Dass SinghMichael Fenech
Mar 31, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Tina Bianco-MiottoClaire Roberts
Jun 14, 2016·Clinical Epigenetics·Masako SuzukiJohn M Greally

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