Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes

AIDS
Tara BeattieS Rowland-Jones

Abstract

The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

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Citations

Jan 13, 2006·Cancer Immunology, Immunotherapy : CII·Judith M RamageLindy G Durrant
May 13, 2009·The Journal of Infectious Diseases·Grace C John-StewartSarah Rowland-Jones
Jun 11, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Ellen MaclachlanAnn Duerr
Jan 1, 2014·The Pediatric Infectious Disease Journal·Lisa M CranmerGrace C John-Stewart
Jul 14, 2006·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Marie NguyenGianfranco Pancino
Jun 4, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kimdar Sherefa KemalHarold Burger
Sep 5, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Lyle R McKinnonFrancis A Plummer
Feb 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M H HammerP Reinke
May 2, 2006·Journal of Immunological Methods·Bartek ZuberJohan K Sandberg
Oct 4, 2011·Journal of Immunological Methods·Mauro S MalnatiHarvey Holmes
Aug 21, 2009·Clinical and Vaccine Immunology : CVI·Xiao-Li HuangCharles R Rinaldo
Dec 14, 2007·Clinical and Vaccine Immunology : CVI·Jeffrey R CurrierJosephine H Cox
Feb 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer N UramElizabeth M Jaffee

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