Screening for petrochemical contamination in seafood by headspace solid-phase microextraction gas chromatography-mass spectrometry

Analytical and Bioanalytical Chemistry
F Aladar BencsathSteven M Plakas

Abstract

A headspace solid-phase microextraction gas chromatography-mass spectrometry (SPME GC-MS) method is described, to screen seafood for volatile organic compounds (VOCs) associated with petrochemical taint. VOCs are extracted from the headspace of heated sample homogenates by adsorption onto a SPME fiber and desorbed for analysis by GC-MS. Targeted compounds are determined semi-quantitatively using representative calibration standards for the various classes (alkanes, alkylbenzenes, indanes/tetralins, and naphthalenes) of VOCs analyzed. Sample preparation is minimal, and the analyses are rapid and automated with a capacity of 50 samples per day. The method was optimized in terms of headspace temperature, sample heating time, extraction time, and desorption time using oyster samples fortified with target compounds. Calibrations for hydrocarbon components were linear in the range of 8.3-167 ng/g; the limit of detection ranged between 0.05 and 0.21 ng/g, and the limit of quantitation between 0.16 and 0.69 ng/g. Good precision (RSD < 10 % at 16.7 ng/g for individual VOCs) and accuracy (recovery range 89-118 % at 25 ng/g) were obtained in oyster, crab, shrimp, and finfish matrices. The trueness of the method was demonstrated by quantif...Continue Reading

References

Aug 1, 1970·Journal of the Science of Food and Agriculture·J ShiptonG L Vale
Feb 13, 2003·Journal of Chromatography. a·Jan C R DemyttenaereNorbert De Kimpe
May 11, 2007·Water Science and Technology : a Journal of the International Association on Water Pollution Research·V RogersB Brownlee
Mar 28, 2013·Journal of Agricultural and Food Chemistry·Susan GenualdiTimothy Begley

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Citations

Oct 4, 2016·Trends in Analytical Chemistry : TRAC·Qihui ZhangChun-Su Yuan
Jun 16, 2016·Marine Pollution Bulletin·Jonny BeyerTracy K Collier

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