PMID: 12758172May 22, 2003Paper

Screening for simian foamy virus infection by using a combined antigen Western blot assay: evidence for a wide distribution among Old World primates and identification of four new divergent viruses

Virology
Althaf I HussainW Switzer

Abstract

Simian foamy viruses (SFVs) belong to a genetically and antigenically diverse class of retroviruses that naturally infect a wide range of nonhuman primates (NHPs) and can also be transmitted to humans occupationally exposed to NHPs. Current serologic detection of SFV infection requires separate Western blot (WB) testing by using two different SFV antigens [SFV(AGM) (African green monkey) and SFV(CPZ) (chimpanzee)]. However, this method is labor intensive and validation is limited to only small numbers of NHPs. To facilitate serologic SFV testing, we developed a WB assay that combines antigens from both SFV(AGM) and SFV(CPZ). The combined-antigen WB (CA-WB) assay was validated with 145 serum samples from 129 NHPs (32 African and Asian species) and 16 humans, all with known SFV infection status determined by PCR. Concordant CA-WB results were obtained for all 145 PCR-positive or -negative primate and human specimens, giving the assay a 100% sensitivity and specificity. In addition, no reactivity was observed in sera from persons positive for human immunodeficiency virus or human T cell lymphotropic virus (HIV/HTLV) (n = 25) or HIV/HTLV-negative U.S. blood donors (n = 100). Using the CA-WB assay, we screened 360 sera from 43 Old W...Continue Reading

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Citations

Mar 18, 2005·Nature·William M SwitzerWalid Heneine
Nov 25, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·P BastoneM Löchelt
Oct 12, 2002·Transfusion·R S BonevaL E Chapman
Jan 11, 2008·AIDS Research and Human Retroviruses·Roumiana S BonevaLouisa E Chapman
Jan 13, 2012·AIDS Research and Human Retroviruses·Fen HuangWeikun Zeng
May 2, 2006·Stem Cells and Development·C Chang I LeeAlice F Tarantal
Jun 26, 2007·Journal of Bacteriology·Fang ChiRegine Hakenbeck
Nov 11, 2011·Journal of Virology·Augustin Mouinga-OndéméMirdad Kazanji
Oct 28, 2004·Journal of Virology·Ernst J VerschoorJonathan L Heeney
Mar 3, 2004·Journal of Virology·William M SwitzerWalid Heneine
Sep 29, 2005·Journal of Virology·James E CumminsCharlene S Dezzutti
Feb 27, 2007·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Hayley Weston MurphyWilliam M Switzer
Jul 19, 2005·Emerging Infectious Diseases·Lisa Jones-EngelJonathan S Allan
Feb 7, 2008·Emerging Infectious Diseases·Sara CalattiniAntoine Gessain
Oct 23, 2015·The Journal of General Virology·Yaqiong WangEdward B Stephens
Aug 11, 2006·American Journal of Primatology·D A TravisL Jones-Engel
Aug 29, 2006·Transfusion·Walid Heneine, Matthew J Kuehnert
Feb 3, 2015·Retrovirology·Gkikas MagiorkinisRobert Belshaw
Apr 25, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ahidjo AyoubaEric Nerrienet
May 8, 2013·Virology·Kimberly SchmittEdward B Stephens
Jun 6, 2015·PLoS Neglected Tropical Diseases·Tierra Smiley EvansChristine K Johnson
Jun 3, 2009·Expert Review of Anti-infective Therapy·Arifa S Khan
Sep 21, 2017·PloS One·Cláudia P MunizWilliam M Switzer

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