Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library: identification of peptide substrates for TGASE 2 and Factor XIIIA

The Journal of Biological Chemistry
Yoshiaki SugimuraKiyotaka Hitomi

Abstract

Mammalian transglutaminase (TGase) catalyzes covalent cross-linking of peptide-bound lysine residues or incorporation of primary amines to limited glutamine residues in substrate proteins. Using an unbiased M13 phage display random peptide library, we developed a screening system to elucidate primary structures surrounding reactive glutamine residue(s) that are preferred by TGase. Screening was performed by selecting phage clones expressing peptides that incorporated biotin-labeled primary amine by the catalytic reactions of TGase 2 and activated Factor XIII (Factor XIIIa). We identified several amino acid sequences that were preferred as glutamine donor substrates, most of which have a marked tendency for individual TGases: TGase 2, QxPphiD(P), QxPphi, and QxxphiDP; Factor XIIIa, QxxphixWP (where x and phi represent a non-conserved and a hydrophobic amino acid, respectively). We further confirmed that the sequences were favored for transamidation using modified glutathione S-transferase (GST) for recombinant peptide-GST fusion proteins. Most of the fusion proteins exhibited a considerable increase in incorporation of primary amines over that of modified GST alone. Furthermore, we identified the amino acid sequences that demons...Continue Reading

References

Apr 15, 1992·European Journal of Biochemistry·P J GroenenW W de Jong
Aug 15, 1992·Analytical Biochemistry·T F SlaughterC S Greenberg
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L LorandP T Velasco
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Dec 5, 1991·Journal of Molecular Biology·J D MarksG Winter
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K N ParameswaranL Lorand
Sep 11, 1999·The International Journal of Biochemistry & Cell Biology·J S Chen, K Mehta
Nov 15, 2000·Methods in Enzymology·S S SidhuJ A Wells
Mar 6, 2002·The Journal of Experimental Medicine·L Willemijn VaderFrits Koning
Sep 5, 2002·Nature Reviews. Immunology·Ludvig M Sollid
Oct 9, 2002·The Biochemical Journal·Martin GriffinCarlo M Bergamini
Oct 9, 2002·Trends in Biochemical Sciences·Laszlo Fesus, Mauro Piacentini
Jan 10, 2003·Nucleic Acids Research·Angelo M FacchianoFrancesco Facchiano
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·Laszlo Lorand, Robert M Graham
May 10, 2003·The Journal of Biological Chemistry·Weijun PanCharles F Albright
May 22, 2003·Applied Microbiology and Biotechnology·Y-C M WangN A Da Silva
May 23, 2003·Protein Science : a Publication of the Protein Society·Margherita RuoppoloCarla Esposito
Mar 2, 2005·The Journal of Investigative Dermatology·Richard L EckertEllen A Rorke
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·Eleonora CandiGerry Melino
Jun 21, 2005·Blood Reviews·Kristina F StandevenPeter J Grant

❮ Previous
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Citations

Jul 4, 2008·Amino Acids·Angelo Facchiano, Francesco Facchiano
May 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zhaoming TangLifang Dou
Jul 27, 2011·Journal of Molecular Neuroscience : MN·Lars DolgeWerner Baumgartner
Aug 24, 2007·Analytical Chemistry·Bi-Huang HuPhillip B Messersmith
Feb 16, 2011·The Journal of Biological Chemistry·Norihisa NishimichiYasuyuki Yokosaki
Feb 11, 2010·Nucleic Acids Research·Roberto Di NiroDaniele Sblattero
Mar 1, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Raymond KwanM Bishr Omary
Jun 12, 2008·Journal of Cell Science·Kristen A JohnsonRobert A Terkeltaub
Sep 30, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Miho ItohKiyotaka Hitomi
Oct 15, 2013·PloS One·Zhengguang GuoYouhe Gao
Feb 18, 2010·Future Neurology·Thomas M JeitnerArthur Jl Cooper
Jan 30, 2013·Analytical Biochemistry·Jon A KennistonAndrew E Nixon
Oct 30, 2010·Current Opinion in Biotechnology·Marlon J Hinner, Kai Johnsson
Aug 4, 2009·Analytical Biochemistry·Kiyotaka HitomiSaïd El Alaoui
Mar 26, 2009·Analytical Biochemistry·Mileidys Perez AleaSaïd El Alaoui
Oct 6, 2007·Advanced Drug Delivery Reviews·Angelo FontanaFrancesco M Veronese
Sep 18, 2007·Biomaterials·Marsha Elizabeth Ritter Jones, Phillip B Messersmith
Nov 1, 2006·Protein Science : a Publication of the Protein Society·Zsolt KeresztessyLászló Fésüs
Nov 15, 2006·Clinical and Experimental Immunology·W SaklyS El Alaoui
Feb 5, 2009·Journal of Thrombosis and Haemostasis : JTH·K PénzesL Muszbek
Sep 22, 2007·Journal of Thrombosis and Haemostasis : JTH·K N LeeP A McKee
Oct 1, 2010·Journal of Thrombosis and Haemostasis : JTH·I KomáromiL Muszbek
Aug 14, 2012·Biotechnology and Bioengineering·Jae-Hun LeeByung-Gee Kim
Oct 19, 2012·Clinical Science·Victoria R RichardsonAngela M Carter

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