Screening for two selective androgen receptor modulators using gas chromatography-mass spectrometry in doping control analysis

European Journal of Mass Spectrometry
M ThevisW Schänzer

Abstract

Selective androgen receptor modulators (SARMs) have become a major field of clinical research enabling the tissue-selective stimulation of androgen receptors. The treatment of debilitating diseases, osteoporosis and frailty are primary goals and promising results have been obtained from clinical trials. However, the potential for misuse of SARMs in sport is great and drug testing methods based on liquid chromatography were established for different classes including arylpropionamide-, 2-quinolinone- and bicyclic hydantoin-derived compounds. As gas chromatography and mass spectrometry (GC-MS) are still important analytical tools in sports drug testing, a method to determine 2-quinolinone- and bicyclic hydantoin-derived SARMs established. Spiked urine samples were subjected to routine doping control protocols including enzymatic hydrolysis, liquid-liquid extraction, concentration and derivatisation to trimethylsilylated analogues followed by GC-MS analysis. The method was validated for the items specificity, lower limit of detection (0.2-10 ng mL(-1)), recovery (83-85%), intraday and interday precision (9-15% and 13-18%, respectively), which demonstrates the suitability of conventional GC-MS systems to determine representatives o...Continue Reading

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Citations

Jun 17, 2010·Wiener klinische Wochenschrift·Karin VitzthumNorman Schöffel
Jul 20, 2010·Annual Review of Analytical Chemistry·Larry D Bowers
Dec 17, 2009·Journal of the American Society for Mass Spectrometry·Laura LuosujärviTapio Kotiaho
Apr 18, 2009·Journal of Mass Spectrometry : JMS·Mario ThevisWilhelm Schänzer
Mar 13, 2009·Rapid Communications in Mass Spectrometry : RCM·Mario ThevisWilhelm Schänzer
Mar 20, 2010·Rapid Communications in Mass Spectrometry : RCM·Andreas ThomasMario Thevis
Jul 28, 2015·Mass Spectrometry Reviews·Wilhelm Schänzer, Mario Thevis

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