Screening for wound-induced oxylipins in Arabidopsis thaliana by differential HPLC-APCI/MS profiling of crude leaf extracts and subsequent characterisation by capillary-scale NMR

Phytochemical Analysis : PCA
Aly ThioconeJean-Luc Wolfender

Abstract

A simple non-targeted differential HPLC-APCI/MS approach has been developed in order to survey metabolome modifications that occur in the leaves of Arabidopsis thaliana following wound-induced stress. The wound-induced accumulation of metabolites, particularly oxylipins, was evaluated by HPLC-MS analysis of crude leaf extracts. A generic, rapid and reproducible pressure liquid extraction procedure was developed for the analysis of restricted leaf samples without the need for specific sample preparation. The presence of various oxylipins was determined by head-to-head comparison of the HPLC-MS data, filtered with a component detection algorithm, and automatically compared with the aid of software searching for small differences in similar HPLC-MS profiles. Repeatability was verified in several specimens belonging to different series. Wound-inducible jasmonates were efficiently highlighted by this non-targeted approach without the need for complex sample preparation as is the case for the 'oxylipin signature' procedure based on GC-MS. Furthermore this HPLC-MS screening technique allowed the isolation of induced compounds for further characterisation by capillary-scale NMR (CapNMR) after HPLC scale-up. In this paper, the screening...Continue Reading

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Citations

Aug 30, 2013·BioMed Research International·Diego F Gomez-CasatiMaría V Busi
Dec 24, 2014·Free Radical Research·G Griffiths
Mar 30, 2010·Angewandte Chemie·Grégoire HervéHervé Graindorge
Apr 21, 2010·Plant Signaling & Behavior·Claus Wasternack, Daoxin Xie
Sep 6, 2008·Phytochemical Analysis : PCA
Nov 11, 2019·International Journal of Molecular Sciences·Fiorenza ProvenzanoDaniela Giacomazza
Sep 9, 2009·Phytochemistry·Cornelia Göbel, Ivo Feussner

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