Screening isolates from antibody phage-display libraries

Drug Discovery Today
David R BucklerRobert Charles Ladner

Abstract

Antibody phage display, coupled with automated screening, facilitates and potentiates the mining of complex combinatorial libraries and the identification of potent drug leads. In managing phage screening data, the behavior of individual phage isolates in binding assays must be linked to their antibody identities as deduced from DNA sequencing. Reviewed here are recently reported approaches for high-throughput screening of clones isolated from phage antibody libraries after selection on a defined antigen. Specific information management challenges, and possible solutions, are described for organizing screening data to enable rapid lead discovery using these antibody libraries.

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Citations

Jun 26, 2009·New Biotechnology·Michael HustStefan Dübel
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
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Jan 12, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Thomas SchirrmannMichael Hust
Mar 10, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hong XuChuanbin Mao

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