Screening method for stimulants in urine by UHPLC-MS/MS: identification of isomeric compounds

Drug Testing and Analysis
N MonfortR Ventura

Abstract

A fast screening method for the detection of more than 60 stimulants in urine was developed. The method consisted of a dilution of the urine (1:5 v/v) and analysis by ultra high performance liquid chromatography coupled to tandem mass spectrometry, using a C18 column (1.7 µm particle size), a mobile phase containing deionized water and acetonitrile with formic acid, and gradient elution. The chromatographic run time was 5 min. The detection was performed in positive mode electrospray ionization, monitoring one or two specific ion transitions for each analyte. Appropriate repeatability was obtained, with relative standard deviation (RSD) values below 25% for most of the analytes. Regarding intermediate precision, estimated during routine work, higher RSDs were obtained, probably due to between-day differences in the status of the mass spectrometer and in the chromatographic system. Matrix effect ranged from 60 to 255% with RSD lower than 35% for the majority of compounds. Despite the matrix effect observed, the signal/noise ratio of the analytes spiked at 50 ng/mL was greater than three in all tested samples, allowing a correct detection of all substances at the minimum required performance levels required by the World Anti-Dopi...Continue Reading

References

Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C JiménezJ Segura
Apr 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Maohe YanJeffrey L Blumer
Sep 5, 2003·Rapid Communications in Mass Spectrometry : RCM·K Deventer, F T Delbeke
Jan 23, 2004·Chemical Society Reviews·Graham J Trout, Rymantas Kazlauskas
Jun 27, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C JiménezR Ventura
May 27, 2008·British Journal of Pharmacology·J R Docherty
Oct 20, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordon J Murray, Jonathan P Danaceau
May 25, 2010·Analytical and Bioanalytical Chemistry·C GómezR Ventura

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Citations

Jan 16, 2016·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Dec 20, 2016·Analytical Chemistry·Vladimir KopysovOleg V Boyarkin
Sep 22, 2020·Analytical and Bioanalytical Chemistry·Claudia BressanRosa Ventura

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