Screening miRNAs associated with resistance gemcitabine from exosomes in A549 lung cancer cells

Cancer Management and Research
Ai-Ling LiangYong-Jun Liu

Abstract

To establish a gemcitabine-resistant lung adenocarcinoma cell line, A549/G+, and to screen the differences of miRNA expression in exosomes from A549 and A549/G+ cells. A549 cells were exposed in gemcitabine until they were resistant to gemcitabine, and extracted exosomes from A549 and A549/G+. The RNAs from exosomes were subjected to miRNA expression microarray experiments. After 39 weeks of continuous induction, we induced drug resistance in A549 cells. The resistance index was 6. Via GeneChip miRNA 4.0 analysis, there were 446 differential miRNAs between A549 and A549/G+. Target gene prediction and pathway analysis discovered the microRNAs in the intersections may participate in drug resistance. These differential miRNAs help to do in-depth research to elucidate the mechanism of resistance to gemcitabine in non-small cell lung cancer.

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

DIANA
microT
miRWalk
Tarbase
ENONTOLOGY
SPSS
Transcriptome Analysis Suite
mirPath
DIANA TOOLS
TargetScan Human

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