Screening molecules for control of citrus huanglongbing using an optimized regeneration system for 'Candidatus Liberibacter asiaticus'-infected periwinkle (Catharanthus roseus) cuttings

Phytopathology
Muqing ZhangCharles A Powell

Abstract

Citrus huanglongbing is one of the most destructive diseases of citrus worldwide. The disease is associated with three different species of 'Candidatus Liberibacter', of which 'Ca. L. asiaticus' is the most widely distributed. An optimized system using 'Ca. L. asiaticus'-infected periwinkle cuttings was developed to screen chemical compounds effective for controlling the bacterial population while simultaneously assessing their phytotoxicity. The optimal regeneration conditions were determined to be the use of vermiculite as a growth medium for the cuttings, and a fertilization routine using half-strength Murashige and Tucker medium supplemented with both naphthalene acetic acid (4 microg/ml) and indole-3-butyric acid (4 microg/ml). This system allowed a plant regeneration rate of 60.6% for 'Ca. L. asiaticus'-infected cuttings in contrast to the <1% regeneration rate with water alone. Two chemical agents, penicillin G sodium and 2,2-dibromo-3-nitrilopropionamide (DBNPA), were found to be effective at eliminating or suppressing the 'Ca. L. asiaticus' bacterium in this periwinkle regeneration system. When treated with penicillin G sodium at 50 microg/ml, all plants regenerated from 'Ca. L. asiaticus'-infected cuttings were 'Ca. L...Continue Reading

References

Jul 1, 1994·International Journal of Systematic Bacteriology·S JagoueixM Garnier
Sep 17, 2005·International Journal of Systematic and Evolutionary Microbiology·Diva do Carmo TeixeiraJoseph Bové
Oct 21, 2005·The Plant Journal : for Cell and Molecular Biology·Hao Chen, Liming Xiong
Jan 6, 2009·Journal of Applied Microbiology·M Curković Perica
Mar 1, 2009·Plant Disease·Lia W LieftingGerard R G Clover
Apr 1, 2009·Plant Disease·George W SundinHerb S Aldwinckle

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Citations

Feb 28, 2013·Phytopathology·Nian Wang, Pankaj Trivedi
Dec 11, 2014·Pest Management Science·Dhana Raj Boina, Jeffrey R Bloomquist
Dec 3, 2017·Microbial Ecology·Shahzad MunirYueqiu He
Jun 7, 2018·Horticulture Research·Fang DingYongping Duan
Feb 16, 2019·Frontiers in Plant Science·Muqing ZhangYongping Duan

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