Screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase

Acta Biologica Hungarica
Ashraf S A El-SayedMohamed I El-Batrik

Abstract

The potency for production of cystathionine γ-lyase (CGL) by the fungal isolates was screened. Among the tested twenty-two isolates, Aspergillus carneus was the potent CGL producer (6.29 U/mg), followed by A. ochraceous (6.03 U/mg), A. versicolor (2.51 U/mg), A. candidus (2.12 U/mg), A. niveus and Penicillium notatum (2.0 U/mg). The potent six isolates producing CGL was characterized morphologically, A. carneus KF723837 was further molecularly characterized based on the sequence of 18S-28S rDNA. Upon sulfur starvation, the yield of A. carneus extracellular CGL was increased by about 1.7- and 4.1-fold comparing to non-sulfur starved and L-methionine free medium, respectively. Also, the uptake of L-methionine was duplicated upon sulfur starvation, assuming the activation of specific transporters for L-methionine and efflux of CGL. Also, the intracellular thiols and GDH activity of A. carneus was strongly increased by S starvation, revealing the activation of in vivo metabolic antioxidant systems. Upon irradiation of A. carneus by 2.0 kGy of γ-rays, the activity of CGL was increased by two-fold, regarding to control, with an obvious decreases on its yield upon further doses. Practically, CGL activity from the solid A. carneus cult...Continue Reading

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Citations

Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Aug 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedMahmoud Z Sitohy
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf El-SayedNashwa El-Gazzar
Oct 8, 2020·Heliyon·Manal T El Sayed, Ashraf S A El-Sayed
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamed RaafatManal T El-Sayed
Jun 9, 2021·Molecular Biology Reports·Marwa M El-DemerdashHala Nosier

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