Screening, mutagenesis and protoplast fusion of Aspergillus niger for the enhancement of extracellular glucose oxidase production

Journal of Industrial Microbiology & Biotechnology
A A Khattab, W A Bazaraa

Abstract

Various strains of Aspergillus niger were screened for extracellular glucose oxidase (GOD) activity. The most effective producer, strain FS-3 (15.9 U mL(-1)), was mutagenized using UV-irradiation or ethyl methane sulfonate. Of the 400 mutants obtained, 32 were found to be resistant to 2-deoxy D: -glucose, and 17 of these exhibited higher GOD activities (from 114.5 to 332.1%) than the original FS-3 strain. Following determination of antifungal resistance of the highest producing mutants, four mutants were selected and used in protoplast fusions in three different intraspecific crosses. All fusants showed higher activities (from 285.5 to 394.2%) than the original strain. Moreover, of the 30 fusants isolated, 19 showed higher GOD activity than their corresponding higher-producing parent strain.

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Citations

Nov 21, 2007·Applied Biochemistry and Biotechnology·Ramida WatanapokasinKohtaro Kirimura
Sep 5, 2008·Applied Biochemistry and Biotechnology·Pardeep Kumar, T Satyanarayana
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Jan 14, 2011·Preparative Biochemistry & Biotechnology·Ozden Canli, Esabi Basaran Kurbanoglu
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Jun 3, 2009·Journal of Applied Microbiology·Y ChengY Qu
May 7, 2013·Bioengineered·Orquídea RibeiroLucília Domingues
Sep 11, 2010·Cognitive Neuropsychology·Yasmeen Faroqi-Shah, Arifi N Waked
May 30, 2019·Biotechnology for Biofuels·Boutheina BessadokSaloua Sadok
Jan 14, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Shijie XuGuangming Yuan

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

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An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.