Screening nested-PCR primer for 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing and application in Hunan, China

PloS One
Yanyun HongTuyong Yi

Abstract

Citrus Huanglongbing (HLB) is one of the most devastating citrus diseases worldwide. Sensitive and accurate assays are vital for efficient prevention of the spread of HLB-associated "Candidatus Liberibacter spp". "Candidatus Liberibacter spp" that infect Citrus includes "Candidatus Liberibacter asiaticus" (Las), "Candidatus Liberibacter africanus" (Laf) and "Candidatus Liberibacter americanus" (Lam). Of them, Las is the most widespread species. In this study, a set of nested PCR primer pairs were screened to diagnose Las, and the nested PCR method greatly enhanced the sensitivity to detect Las up to 10 times and 100 times compared to qPCR and conventional PCR, respectively. Totally, 1112 samples from 5 different citrus cultivars in 39 different counties and cities were assayed by nested PCR. The results show that 384 samples were HLB-infected; the highest positive detection rate was 79.7% from the lopsided fruit samples, and the lowest positive detection rate was 16.3% from the apical dieback samples. The results indicate that the designed nested PCR primer pairs can detect Las from different symptomatic tissues, different citrus cultivars and different geographic regions. The set of nested PCR primers designed in the present s...Continue Reading

References

Aug 20, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·T P AndersonD R Murdoch
Nov 5, 2005·Applied and Environmental Microbiology·C BastianelS Eveillard
Sep 7, 2007·Journal of Clinical Microbiology·W Y LamPaul K S Chan
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Yongping DuanTim Gottwald
Apr 27, 2010·Annual Review of Phytopathology·Tim R Gottwald
Nov 16, 2014·BMC Microbiology·Getachew Tadesse, Tesfaye S Tessema
Sep 4, 2016·Canadian Journal of Microbiology·Roberto Arredondo ValdésYisa M Ochoa Fuentes

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Citations

Oct 15, 2021·Phytopathology·Berta AlquézarLeandro Peña

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Datasets Mentioned

BETA
KY849808.1
X55749.1

Methods Mentioned

BETA
PCR
electrophoresis
enzyme-linked immunosorbent assay

Software Mentioned

Beacon Design
BLAST

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