Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS

Journal of Analytical Toxicology
Kelly Francisco da CunhaJose Luiz Costa

Abstract

New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines, and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid elution buffer mixture (500 μL sample) was extracted with t-butyl methyl ether (MTBE), and chromatographic separation was performed on a Raptor™ biphenyl column (100 mm x 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally less than 20% and overall extraction recoveries greater than 60%. The highest matrix effect was observed within the synthetic cannabinoids group (PB-22, -55.5%). Lower recoveries were observed for 2C-T (47....Continue Reading

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Jul 29, 2018·Drug Testing and Analysis·Lilian de Melo BarbosaJose Luiz Costa
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Citations

Jan 23, 2021·The American Journal of Drug and Alcohol Abuse·Stephen MaguraPiyadarsha Amaratunga
Feb 14, 2021·Journal of Pharmaceutical and Biomedical Analysis·Júlia Martinelli Magalhães KahlJose Luiz Costa
Mar 19, 2021·Forensic Science International : Synergy·Aslı AtasoySerap Annette Akgur
Jul 22, 2021·Analytical and Bioanalytical Chemistry·Ángela López-RabuñalAlberto Salomone
Jul 22, 2021·Journal of Forensic Sciences·Kaitlyn B Palmquist, Madeleine J Swortwood

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