Screening of a thiamine-auxotrophic yeast for alpha-ketoglutaric acid overproduction

Letters in Applied Microbiology
Jingwen ZhouJian Chen

Abstract

To obtain a thiamine-auxotrophic yeast strain that overproduces alpha-ketoglutaric acid (alpha-KG) from glycerol and to investigate nutrient effects on alpha-KG production. Yeast strain WSH-Z06, a thiamine auxotroph that gave high yields of alpha-KG from glycerol, was obtained by screening for ampicillin/kanamycin resistance and thiamine auxotrophy. The strain was identified as Yarrowia lipolytica based on physiological, chemical, and phylogenetic analysis. The ability of the strain to convert glycerol to alpha-KG was analysed by investigating the effects of nutritional factors, including thiamine, riboflavin, nitrogen sources, and calcium ion. Thiamine and calcium ion concentration had the greatest effect on alpha-KG accumulation. Under optimal conditions, a yield of 39.2 g l(-1)alpha-KG was obtained from 100 g l(-1) glycerol, with 16.84 g l(-1) pyruvate as a by-product. The current work provides a method for screening for an alpha-KG overproducer. Nutrients have a significant impact on alpha-KG production in the yeast strain presented here. The alpha-KG-overproducing yeast strain Y. lipolytica WSH-Z06 is a promising parent strain for further metabolic engineering to lower by-product accumulation and accelerate glycerol utiliz...Continue Reading

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Citations

Oct 4, 2011·Applied Microbiology and Biotechnology·Christina OttoGerold Barth
Feb 26, 2013·Applied Microbiology and Biotechnology·Svetlana V Kamzolova, Igor G Morgunov
Aug 21, 2013·Applied Microbiology and Biotechnology·Igor G MorgunovVladimir A Samoilenko
Jun 29, 2014·Applied Microbiology and Biotechnology·Svetlana V KamzolovaIgor G Morgunov
Apr 22, 2016·Bioprocess and Biosystems Engineering·Mei-Juan TanZhen-Ming Chi
Jul 9, 2016·International Journal of Food Microbiology·Weng Chan VongShao-Quan Liu
Jan 31, 2015·Scientific Reports·Hongwei GuoJian Chen
Oct 4, 2015·Current Opinion in Biotechnology·Jason T BoockKristala Lj Prather
Mar 25, 2014·Journal of Biotechnology·Panqing NiuLiming Liu
Mar 20, 2019·Folia Microbiologica·Krzysztof CybulskiAnita Rywińska
Jan 4, 2017·Applied Microbiology and Biotechnology·Igor G MorgunovMikhail B Vainshtein
Jul 13, 2018·Bioprocess and Biosystems Engineering·Weizhu ZengJingwen Zhou
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen
Feb 7, 2021·Biotechnology Advances·Zhengshan LuoJingwen Zhou
Jun 9, 2018·Trends in Biotechnology·Kelly A Markham, Hal S Alper

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