Screening of bacterial endophytes as potential biocontrol agents against soybean diseases

Journal of Applied Microbiology
K B de Almeida LopesG Degrassi

Abstract

This research was aimed at identifying and characterizing endophytic micro-organisms associated with soybean that have antimicrobial activity towards soybean pathogens. Soybean plants were collected from field trials in four locations of southern Brazil that were cultivated with conventional (C) and transgenic glyphosate-resistant (GR) soybeans. Endophytic bacteria isolated from roots, stems and leaves of soybeans were evaluated for their capacity to inhibit fungal and bacterial plant pathogens and 13 micro-organisms were identified with antagonistic activity. Approximately 230 bacteria were isolated and identified based on the 16S rRNA and rpoN gene sequences. Bacteria isolated from conventional and transgenic soybeans were significantly different not only in population diversity but also in their antagonistic capacity. Thirteen isolates showed in vitro antagonism against Sclerotinia sclerotiorum, Phomopsis sojae and Rhizoctonia solani. Bacillus sp. and Burkholderia sp. were the most effective isolates in controlling bacterial and fungal pathogens in vitro. Extracts and precipitates from culture supernatants of isolates showed different patterns of inhibitory activity on growth of fungal and bacterial pathogens. Bacillus sp. a...Continue Reading

References

Apr 1, 1997·International Journal of Systematic Bacteriology·Y AnzaiH Oyaizu
Apr 27, 2002·Applied and Environmental Microbiology·Denise K ZinnielAnne K Vidaver
May 22, 2003·Microbes and Infection·Gary A Strobel
Nov 25, 2004·Environmental Microbiology·Júlia Kuklinsky-SobralJoão Lúcio Azevedo
Aug 5, 2005·Annual Review of Phytopathology·D R Fravel
Mar 21, 2007·FEMS Microbiology Letters·Emilio Montesinos
Apr 13, 2007·Molecular Plant-microbe Interactions : MPMI·Diego RomeroAlejandro Perez-Garcia
Nov 24, 2007·FEMS Microbiology Letters·Robert P RyanDavid N Dowling
Dec 9, 2008·Applied and Environmental Microbiology·Safiyh TaghaviDaniel van der Lelie
Oct 19, 2010·Journal of Bacteriology·Theo H M SmitsBrion Duffy
May 10, 2011·Science·Rodrigo MendesJos M Raaijmakers
Sep 29, 2011·Prikladnaia biokhimiia i mikrobiologiia·I V MaksimovL I Pusenkova
Mar 3, 2015·Journal of Basic Microbiology·Akansha JainHarikesh Bahadur Singh
May 20, 2015·World Journal of Microbiology & Biotechnology·Bihong ShiXiaolei Luo
Jun 6, 2015·Genome Announcements·Xianfa MengVittorio Venturi
Jan 9, 2016·Journal of Applied Microbiology·K B de Almeida LopesG Degrassi
Apr 3, 2016·Applied Microbiology and Biotechnology·Panagiotis Chanos, Tina Mygind
Apr 6, 2016·International Journal of Systematic and Evolutionary Microbiology·Chun Yan LiChun Tao Gu
Jul 8, 2017·Applied Microbiology and Biotechnology·Haobin ZhaoMingliang Jin
Jul 15, 2017·Frontiers in Plant Science·Jegadeesan RamalingamRanganathan Chandrababu
Jan 25, 2018·Plant Science : an International Journal of Experimental Plant Biology·Sharifah Farhana Syed Ab RahmanPeer M Schenk
Apr 1, 2016·Journal of Pharmaceutical Analysis·Mounyr BalouiriSaad Koraichi Ibnsouda
Apr 18, 2018·Frontiers in Microbiology·David Correa-GaleoteGregorio J Arone

Citations

Dec 10, 2020·International Journal of Molecular Sciences·Yee-Shan KuHon-Ming Lam

Related Concepts

Cochliobolus
Hyphomycetes
Pest Control, Biological
Plant Diseases
Rhizoctonia
RNA, Ribosomal, 16S
Glycine max
Bacterial Physiological Phenomena
Plant Leaves
Plant bulb

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