Screening of by-products from the food industry as growth promoting agents in serum-free media for skeletal muscle cell culture

Food & Function
R Christel AndreassenSissel Beate Rønning

Abstract

The most significant cost driver for efficient bio-production of edible animal proteins is the cell culture media, where growth factors account for up to 96% of the total cost. The culture media must be serum-free, affordable, contain only food-grade ingredients, be efficient to promote cell growth and available in massive quantities. The commercially available serum substitutes are expensive and not necessarily food-grade. Identifying inexpensive food-safe alternatives to serum is crucial. By-products from food production are available in massive quantities, contain potential factors that can promote growth and are promising ingredients for serum replacement. The main goal of this study was to explore if food-grade by-product materials can be used as growth promoting agents in skeletal muscle cell culture to develop a tailor-made serum free media. Different by-products, including chicken carcass, cod backbone, eggshell membrane, egg white powder and pork plasma were enzymatically or chemically hydrolyzed. The hydrolysates in addition to lyophilized pork plasma and yeast extract were further characterized by size-exclusion chromatography, elemental combustion analysis and degree of hydrolysis. The materials were used as supplem...Continue Reading

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Citations

Sep 22, 2020·Critical Reviews in Food Science and Nutrition·Harish K HandralDeepak Choudhury
Dec 17, 2020·Comprehensive Reviews in Food Science and Food Safety·Edward N O'NeillDavid E Block

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Methods Mentioned

BETA
biopsy
Size exclusion chromatography
Assay

Software Mentioned

Graph Pad Prism
[UNK] Chromatography Data System ( CDS )
GraphPadSoftware

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