Screening of cardioprotective diseases bioactive components from Danshen extracts and LC-MS analysis.

Biomedical Chromatography : BMC
Jiang-Feng YuanDa-Hong Wang

Abstract

A rapid and efficient analysis and screening method is adopted for cell affinity capture coupled with HPLC-MS (CAC-HPLC-MS) analysis of bioactive components that have possible efficiency against cardiovascular diseases. This method involves affinity capture, concentration, and separation of bioactive components from Danshen library using oxidatively damaged endothelial cells induced by H2 O2 , as well as analysis and identification of targeted compounds with HPLC and MS. It combines the specific interaction between cell membrane receptors and bioactive components with the powerful analysis and identification function of HPLC-MS. The CAC-HPLC-MS method was also used for analysis and screening of bioactive components from crude extracts of Danshen. A total of 19 components were found to be bound to oxidatively damaged endothelial cells with seven of these identified. Existing literature confirms that these seven components have many activities related to cardioprotective diseases. Therefore, the combination of biological affinity capture with HPLC-MS should be regarded as an attractive method with great potential for rapid and efficient screening of bioactive components related to anti-cardiovascular diseases from natural product...Continue Reading

References

May 17, 2000·Proteins·F FogolariH Molinari
Jun 9, 2004·Anesthesiology Clinics of North America·Dean T Giacobbe, Michael J Murray
Dec 29, 2004·Journal of Pharmaceutical and Biomedical Analysis·Jin-lan ZhangDe-an Guo
Oct 6, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhi-Xiong HuShi-Lu Da
Nov 19, 2008·Biomedical Chromatography : BMC·Jiang-Feng YuanJian-Li Liu
Dec 11, 2008·Basic & Clinical Pharmacology & Toxicology·Guang-De YangQiao-Li Ji
Dec 17, 2008·Combinatorial Chemistry & High Throughput Screening·Daniel J UrbanChristopher P Austin
Jul 14, 2010·Antioxidants & Redox Signaling·Young-Myeong KimHun-Taeg Chung
Mar 29, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Qinghua ShangLi Huang
Mar 13, 2013·International Journal of Molecular Sciences·Junni LiuFan Jiang
Jun 20, 2013·Microvascular Research·Valeria ZagoLaura Schreier
Jul 31, 2014·The Journal of Endocrinology·Yoswaris SemamingNipon Chattipakorn
Jul 31, 2014·Journal of Pharmacological Sciences·Xi-Lan TangJin-Cai Hou
Dec 3, 2014·Computational Biology and Chemistry·Zahida ParveenSajid Rashid
Mar 26, 2015·Mechanisms of Ageing and Development·Daniela GradinaruGabriel Ioan Prada
Aug 19, 2015·Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·Cui-ying ZhangHeng-wen Chen
Jan 7, 2016·The Journal of Physiological Sciences : JPS·Zuraini AhmadMuhammad Nazrul Hakim
Feb 2, 2019·Chinese Journal of Natural Medicines·Xiao-Dan MEImJia-Yu Zhang
Mar 5, 2019·Biochimica Et Biophysica Acta. Biomembranes·Yoshiaki Yano, Katsumi Matsuzaki
Apr 10, 2020·Revista Brasileira De Medicina Do Trabalho : Publicação Oficial Da Associação Nacional De Medicina Do Trabalho-ANAMT·Frida Marina Fischer, Elizabeth Costa Dias

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