Screening of endophytic bacteria against fungal plant pathogens

Journal of Environmental Sciences (China)
Tatsuya OhikeTakashi Ano

Abstract

Bacterial endophytes were found from 6 plant leaves among 35 plant leaves screened. Two of the isolated bacteria showed antagonistic activity against fungal plant pathogens. An isolate named KL1 showed the clear inihibition against plant pathogens, Fusarium oxysporum and Rhizoctonia solani, on PDA as well as TSA plate. Supernatant of the bacterial culture also showed the clear inhibition against the fungal growth on the plate and the antibiotic substance was identified as iturin A by HPLC analysis. KL1 was identified as Bacillus sp. from the 16S rRNA gene analysis. Very thin hyphae of R. solani was miccroscopically observed when the fungus was co-cultivated with KL1.

Related Concepts

High Pressure Liquid Chromatography Procedure
Filamentous fungus
Plant Leaves
Lipopeptides
Endophytes
Antibiotics
Fungi
Iturin A
Trichostatin A
Antagonists

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