PMID: 15385738Sep 24, 2004Paper

Screening of HIV-1 isolates by reverse heteroduplex mobility assay and identification of non-B subtypes in Italy

Journal of Acquired Immune Deficiency Syndromes : JAIDS
L BuonaguroItalian Concerted Action on PHI Treatment

Abstract

The increasing prevalence of HIV-1 transmission through heterosexual contacts and the growing number of immigrants from non-Western countries, where non-B subtypes and recombinant forms are prevalent, suggest the possible emergence in Italy of a new epidemic wave of HIV-1 non-B subtypes as well as recombinant forms. The distribution of HIV-1 subtypes has been evaluated in 63 seropositive individuals residing in Italy, most of whom were infected through a sexual route during the last 5 years. A modified heteroduplex mobility assay (HMA) strategy, reverse HMA (rHMA), has been developed in our laboratory, allowing rapid identification of divergent-from-B-subtype isolates, which have been subsequently characterized by detailed molecular and phylogenetic analyses. Five samples show, on rHMA, an electrophoretic pattern compatible with a non-B subtype classification. Their phylogenetic analysis, performed on both env and gag regions, confirms the rHMA subtyping prediction, given that 3 samples fall into the "A-family" subtype and 2 into the G subtype. The 5 non-B-subtype HIV-1 isolates have been identified among 23 variants (prevalence, 21.74%) isolated during the 2000 to 2001 period in heterosexuals. In parallel, B-subtype isolates s...Continue Reading

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Citations

Oct 28, 2006·AIDS Research and Human Retroviruses·M TagliamonteL Buonaguro
Dec 26, 2008·AIDS Research and Human Retroviruses·M GiulianiM Ciccozzi
Oct 14, 2008·Infectious Agents and Cancer·Luigi BuonaguroFranco M Buonaguro
Dec 27, 2013·BioMed Research International·Massimo GiulianiMassimo Ciccozzi
Sep 3, 2013·Journal of Virological Methods·Raj Shankarappa, James I Mullins
Oct 13, 2012·Journal of Virological Methods·Renée M van der SluisGeorgios Pollakis
Apr 4, 2006·Revista de saúde pública·Henry I Z Requejo
May 16, 2007·AIDS·Massimo CiccozziGiovanni Rezza
Jul 23, 2009·Expert Review of Anti-infective Therapy·Giordano MadedduMaria Stella Mura
Feb 6, 2020·Epidemiology and Infection·Caterina SagnelliEvangelista Sagnelli

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