Screening of monoclonal antibodies using antigens labeled with acetylcholinesterase

Methods in Molecular Biology
Y Frobert, J Grassi

Abstract

The production of large quantities of monoclonal antibodies (MAbs) of predetermined specificity has been rendered possible by the pioneering work of Köhler and Milstein (1). These workers have shown that lymphocytes can be immortalized and subsequently cultured after somatic fusion with genetically selected myeloma cells. Usually, once fusion between spleen cells and myeloma cells has been performed, cells are suspended in a large volume of selective medium and distributed in culture wells, so that hybridomas are brought to clonal dilution. If fusion is successful, the first hybridoma colonies will be detectable within a few days (5-15 d). As fusion is a random process, most clones code for MAbs of unknown specificity, characterizing the immunological past of the host. It is then necessary to select the different colonies that secrete MAbs of the desired specificity. Owing to the great number of wells to be tested (often a few hundred), and to the small quantities of MAbs available (at best, 300 µL at a few µg/mL), it is not easy at this stage to characterize the fine specificity of the antibodies (i.e., recognition of a precise epitope, inhibitory effect on a biological system, properties suitable for purifying antigen, or for...Continue Reading

Related Concepts

Related Feeds