Screening of organ and tissue donors for West Nile virus by nucleic acid amplification--a three year experience in Alberta

American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Peter TilleyJutta K Preiksaitis

Abstract

West Nile Virus (WNV)-specific nucleic acid amplification testing (NAAT) of organ and tissue donors remains controversial. We report three years of WNV donor screening in Alberta Canada using NAAT. Between 2003 and 2005, 1549 initial specimens were received. A valid negative result was issued within the specified turnaround time on 1531 (98.8%). The initial NAAT was successful for 1393 samples (90%), while repeat testing using an alternate NAAT resolved a further 126 samples. For 12 of 14 donors, a second specimen provided a valid negative result. Failure to generate a valid negative result in time resulted in rescheduling of one living related organ transplant, and surgery proceeded in the absence of a final result in one multi-organ donation after risk assessment. For 11 tissue donors, tissues were discarded due to lack of a WNV result. Invalid results usually occurred on postmortem haemolyzed tissue donor samples due to inhibitory reactions. There were no confirmed positive donors, no false-positive results and no solid organs lost due to WNV testing. We conclude that WNV NAAT of organ and tissue donors can be implemented without compromising availability of donors but requires committed laboratory support.

References

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Apr 19, 2006·The Journal of Infectious Diseases·Peter A G TilleyJutta K Preiksaitis

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Citations

Oct 20, 2011·Epidemiology and Infection·S ChinikarS M Ghiasi
Jan 18, 2011·Transplant Infectious Disease : an Official Journal of the Transplantation Society·C RheeB G Blackburn
May 23, 2012·Transplant Infectious Disease : an Official Journal of the Transplantation Society·R J NettJ E Staples
Nov 20, 2016·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Amanda Rosen, Michael G Ison
Feb 27, 2016·F1000Research·Evandro R WinkelmannTian Wang
May 7, 2010·Current Opinion in Organ Transplantation·Rebecca Pellett Madan, Betsy C Herold
Nov 26, 2010·Nursing Ethics·Hakan ErtinIbrahim Basagaoglu
Jan 1, 2015·Emerging Microbes & Infections·Manisha A KulkarniNicholas H Ogden

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