Screening of polyacetylenic alcohols in crude drugs using the ELISA for panaxytriol

Biological & Pharmaceutical Bulletin
T SaitaM Mori

Abstract

Polyacetylenic alcohols such as panaxytriol, panaxynol and panaxydol isolated from the roots of Panax ginseng C. A. MEYER have antiproliferative activity against various cultured tumor cells. Anti-panaxytriol antibody was obtained by immunizing rabbits with panaxytriol-bovine serum albumin conjugates. Although the antibody reactivity was directed mainly toward panaxytriol, there was a slight cross-reactivity with other polyacetylenic compounds. The antibody was, therefore, used for screening a large number of crude drugs for polyacetylenic compounds such as panaxytriol. Methanol-extracts from 31 crude drugs were examined. Significant reactivity was observed in 15 methanol-extracts from Aralieaceae, Compositae and Umbelliferae as reported by other investigators. Three out of the 15 crude drugs were selected for determination of the potent cross-reactive compounds. Four kinds of cross-reactive compounds were isolated by silica gel column chromatography, monitoring each fraction using the enzyme-linked immunosorbent assay (ELISA). Among them, panaxynol and heptadeca-1,8-diene-4,6-diyne-3,10-diol were identified from Saposhnikoviae Radix. Falcarindiol was newly identified from Peucedani Radix. A new polyacetylenic alcohol, 9,10-epo...Continue Reading

Citations

Jul 23, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Dmitry V KuklevValery M Dembitsky
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ting-Chao ChouSamuel J Danishefsky
Sep 7, 2014·Progress in Lipid Research·Dmitry V Kuklev, Valery M Dembitsky
Jan 28, 2006·Assay and Drug Development Technologies·Kevin Yi-Lwern YapChu Sing Lim
Jan 20, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Zhuo LiuDongxin Wang
Dec 6, 2005·The Journal of Organic Chemistry·Heedong YunSamuel J Danishefsky

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