Screening of polyhydroxyalkanoate-producing bacteria and PhaC-encoding genes in two hypersaline microbial mats from Guerrero Negro, Baja California Sur, Mexico

PeerJ
Carolina A Martínez-GutiérrezAlejandro López-Cortés

Abstract

Hypersaline microbial mats develop through seasonal and diel fluctuations, as well as under several physicochemical variables. Hence, resident microorganisms commonly employ strategies such as the synthesis of polyhydroxyalkanoates (PHAs) in order to resist changing and stressful conditions. However, the knowledge of bacterial PHA production in hypersaline microbial mats has been limited to date, particularly in regard to medium-chain length PHAs (mcl-PHAs), which have biotechnological applications due to their plastic properties. The aim of this study was to obtain evidence for PHA production in two hypersaline microbial mats of Guerrero Negro, Mexico by searching for PHA granules and PHA synthase genes in isolated bacterial strains and environmental samples. Six PHA-producing strains were identified by 16S rRNA gene sequencing; three of them corresponded to a Halomonas sp. In addition, Paracoccus sp., Planomicrobium sp. and Staphylococcus sp. were also identified as PHA producers. Presumptive PHA granules and PHA synthases genes were detected in both sampling sites. Moreover, phylogenetic analysis showed that most of the phylotypes were distantly related to putative PhaC synthases class I sequences belonging to members of the...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1973·Advances in Microbial Physiology·E A Dawes, P J Senior
Jan 1, 1993·Bio Systems·R GuerreroG Rampone
Dec 1, 1996·Computer Applications in the Biosciences : CABIOS·N GaltierC Gautier
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Mar 28, 2002·Applied and Environmental Microbiology·Andreas TeskeHolger W Jannasch
Sep 5, 2003·The Biochemical Journal·Bernd H A Rehm
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Jul 1, 2005·Critical Reviews in Microbiology·Daniel KadouriSusana Castro-Sowinski
May 5, 2006·Applied and Environmental Microbiology·Ruth E LeyNorman R Pace
Jun 21, 2007·Journal of Applied Microbiology·R A J VerlindenI Radecka
Oct 30, 2007·Microbial Ecology·Alejandro López-CortésJosé Q García-Maldonado
May 5, 2010·Nucleic Acids Research·Mickael GoujonRodrigo Lopez
Jun 29, 2010·Nature Reviews. Microbiology·Bernd H A Rehm
Jul 14, 2010·FEMS Microbiology Ecology·Laura VillanuevaRicardo Guerrero
Feb 22, 2011·Bioinformatics·Diego DarribaDavid Posada
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Nov 30, 2012·The ISME Journal·Luke C BurowSteven W Singer
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Oct 5, 2014·Extremophiles : Life Under Extreme Conditions·Katariina PärnänenHermanni Kaartokallio

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Datasets Mentioned

BETA
MG201835
MG652451

Methods Mentioned

BETA
electrophoresis
PCR
Transeq

Software Mentioned

MEGA
ProtTest
HIT
SeaView
OligoAnalyzer
PhyML
Genewiz
CD
BLAST
CodonCode Aligner

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