Screening of red blood cells for extracellular vesicle content as a product quality indicator

Transfusion
Jason P AckerElisabeth Maurer-Spurej

Abstract

The controversy around the quality and clinical impact of stored and differentially manufactured red cell concentrates (RCCs) from different donor groups is ongoing. Current studies are limited by the lack of quality measures suitable for routine screening of RCCs. As extracellular vesicles (EVs) are markers of cellular activation or degradation, this study investigated the utility of EV screening to characterize the effects of RBCs production methods and storage. RCCs were prepared by whole blood filtration or red blood cell (RBC) filtration methods, centrifuged to prepare a supernatant, and tested for EV content (dynamic light scattering or tunable resistive pulse-sensing techniques), hemolysis, ATP, and RBC deformability on Days 7, 21, and 42 of storage. To simulate nondestructive quality control (QC) testing, 1 RBC unit was tested in parallel with six 10-mL aliquots that were stored in small-volume containers. EV content showed a linear increase with storage time (p < 0.001) and correlated with supernatant hemoglobin and inversely with ATP or RBC deformability. The method of component manufacturing influenced the characteristics of the EVs during storage. A strong correlation between both EV testing methods' measure of tota...Continue Reading

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Nov 21, 2016·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Ruqayyah J AlmizraqJason P Acker
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Citations

Apr 18, 2020·The Journal of Trauma and Acute Care Surgery·Joo-Yeun OhRakesh P Patel
May 1, 2021·International Journal of Molecular Sciences·Abdelnaby Khalyfa, David Sanz-Rubio
Mar 24, 2021·Proteomics·Lidia Hermida-Nogueira, Ángel García
Jun 1, 2021·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Vassilis L TzounakasMarianna H Antonelou

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