Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry

Biotechnology Letters
Ditte BungertAndreas Tholey

Abstract

Quantitative matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) was applied for the screening of ten pyranose oxidase variants. Quantitative MALDI-ToF MS using isotopic labeled internal standards and ionic liquid matrices was performed using aliquots of enzyme reaction mixtures without prepurification steps. The results obtained were in good agreement with HPLC measurements. Analysis time was approx. 3.5 min for a five-fold determination. Thus, quantitative MALDI-ToF MS can be used as a tool for screening of sugar converting enzymes.

Citations

Jul 12, 2005·Analytical and Bioanalytical Chemistry·André Liesener, Uwe Karst
Jul 11, 2006·Analytical and Bioanalytical Chemistry·Andreas Tholey, Elmar Heinzle
Jul 8, 2010·Analytical and Bioanalytical Chemistry·Estela GiménezVictoria Sanz-Nebot
Mar 22, 2014·Analytical and Bioanalytical Chemistry·Klaus Dreisewerd
Aug 19, 2014·Current Opinion in Biotechnology·Tristan de RondTrent Northen
Mar 30, 2006·Journal of Biotechnology·Dorothée M Heckmann-PohlIris Antes
May 5, 2009·Rapid Communications in Mass Spectrometry : RCM·Cosima D CalvanoFrancesco Palmisano
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Feb 13, 2010·Journal of Proteome Research·Mark W TowersRainer Cramer
Oct 30, 2010·Analytical Chemistry·Nicolas Smargiasso, Edwin De Pauw

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