Screening of Taxol-producing fungi based on PCR amplification from Taxus

Biotechnology Letters
Peng ZhangLong-Jiang Yu

Abstract

Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.

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Citations

Jul 16, 2010·The Journal of Antibiotics·Zoila R Flores-BustamanteLuis B Flores-Cotera
Jul 17, 2010·Journal of Bioscience and Bioengineering·Rangarajulu Senthil KumaranByung-Ki Hur
Oct 28, 2016·Indian Journal of Microbiology·M VasundharaAnil Kumar
May 6, 2019·World Journal of Microbiology & Biotechnology·Harman Gill, M Vasundhara
Jan 18, 2012·Natural Product Reports·Xiao-Long YangDu-Qiang Luo
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Feb 4, 2021·Journal of Natural Products·Sharna-Kay Daley, Geoffrey A Cordell

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