Screening of thermotolerant and thermophilic fungi aiming β-xylosidase and arabinanase production

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
Vivian Machado BenassiMaria de Lourdes Teixeira de Moraes Polizeli

Abstract

Plant cell wall is mainly composed by cellulose, hemicellulose and lignin. The heterogeneous structure and composition of the hemicellulose are key impediments to its depolymerization and subsequent use in fermentation processes. Thus, this study aimed to perform a screening of thermophilic and thermotolerant filamentous fungi collected from different regions of the São Paulo state, and analyze the production of β-xylosidase and arabinanase at different temperatures. These enzymes are important to cell wall degradation and synthesis of end products as xylose and arabinose, respectively, which are significant sugars to fermentation and ethanol production. A total of 12 fungal species were analyzed and 9 of them grew at 45 °C, suggesting a thermophilic or thermotolerant character. Additionally Aspergillus thermomutatus anamorph of Neosartorya and A. parasiticus grew at 50 °C. Aspergillus niger and Aspergillus thermomutatus were the filamentous fungi with the most expressive production of β-xylosidase and arabinanase, respectively. In general for most of the tested microorganisms, β-xylosidase and arabinanase activities from mycelial extract (intracellular form) were higher in cultures grown at high temperatures (35-40 °C), while ...Continue Reading

Citations

Jun 4, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Juliana Moço CorrêaRita de Cássia Garcia Simão
Nov 8, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mario Lucio LopesHenrique Vianna de Amorim

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BETA
Light Microscopy

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