Screening of virulence-associated genes as a molecular typing method for characterization of Streptococcus suis isolates recovered from wild boars and pigs

The Veterinary Journal
V Sánchez del ReyA I Vela

Abstract

Streptococcus suis is an important zoonotic pathogen associated with a wide range of diseases in pigs, but has also been isolated from wild animals such as rabbits and wild boars. In the current study, 126 S. suis isolates recovered from pigs (n = 85) and wild boars (n = 41) were tested by polymerase chain reaction (PCR) for the presence of nine virulence-associated genes. S. suis isolates from wild boars were differentiated by the lower detection rates of the epf, sly, mrp, sao and dltA genes (0%, 2.4%, 2.4%, 4.8% and 21.9%, respectively) compared with the isolates from pigs (56.5%, 75.3%, 56.5%, 88.2.0% and 88.2%, respectively). The differences in the content of these virulence-associated genes were statistically significant (P < 0.05). There was a correlation between the variants saoM and saoL and serotypes 2 and 9, respectively (P < 0.05). Isolates were classified into 31 virulence-associated gene profiles (VPs). Ten VPs were detected among wild boar isolates and 22 VPs among pig isolates, with only two VPs common to wild boars and pigs. The predominant VPs among isolates from wild boars (VP1, VP7) were different from those observed in pig isolates (VP16 and VP26). VP16 was detected exclusively in clinical pig isolates of s...Continue Reading

References

Jun 7, 2003·Journal of Clinical Microbiology·Ana I VelaJosé F Fernández-Garayzábal
Jan 25, 2006·Veterinary Microbiology·Luciana M G SilvaPeter Valentin-Weigand
Nov 7, 2006·Applied and Environmental Microbiology·Christoph G BaumsPeter Valentin-Weigand
Aug 19, 2007·Animal Health Research Reviews·Marcelo GottschalkJiangu Xu
Mar 10, 2010·Future Microbiology·Marcelo GottschalkMariela Segura
Dec 17, 2011·Emerging Infectious Diseases·Nahuel FittipaldiMarcelo Gottschalk
Apr 3, 2013·Journal of Microbiological Methods·Forough L NowrouzianChristina Ahrén
Jun 4, 2013·Veterinary Microbiology·V Sánchez del ReyA I Vela
Apr 15, 2014·The Veterinary Journal·Verónica Sánchez del ReyAna Isabel Vela

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Citations

Mar 31, 2018·Microbiology Spectrum·Marisa HaenniJean-Yves Madec

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