Screening Platform Based on Robolid Microplate for Immobilized Enzyme-Based Assays

ACS Omega
Jiqing YangLi Yang

Abstract

A facile, cost-effective, and high-throughput screening method was developed for enzyme-based assays based on Robolid/Microplate (RLMP) platform. The RLMP platform is constructed by immobilizing enzyme on commercial robolids and combining it with a standard 96-well microplate to achieve high-throughput analysis. The initiation and quenching of enzymatic reaction can be performed by simply sandwiching or unsealing the enzyme-immobilized robolids and the sample-containing microplate. This platform enables measurements of multiple target analytes simultaneously based on immobilized enzymatic reactions, with analysis time independent of the number of wells in the microplate. Using urea as the model analyte, we have shown that the RLMP platform exhibits large linear detection range of up to 10 mM, fast analysis time of 30 min/96 samples, as well as good reproducibility and stability. Measurements of urea in human urine and serum samples were performed using the RLMP platform and were compared with the commercial urea test kit. A good correlation was found between the two methods. This study shows that the present RLMP platform has promising prospects for detection of clinical markers and application in disease diagnosis and biochemi...Continue Reading

References

Jun 16, 2009·Journal of Biomolecular Screening·Sumitra M SukumaranJonathan S Dordick
Oct 14, 2010·Analytical Chemistry·Maria J Ruedas-Rama, Elizabeth A H Hall
Apr 12, 2011·Analytical Chemistry·Cheong Hoon KwonAli Khademhosseini
Sep 2, 2011·Journal of the American Veterinary Medical Association·Emily F HaggettPhilip H Kass
Aug 4, 2012·Biosensors & Bioelectronics·Elisabet Prats-AlfonsoEva Baldrich
Mar 28, 2013·Chemical Society Reviews·Roger A Sheldon, Sander van Pelt
Nov 26, 2015·Analytical Chemistry·María Moreno-GuzmánAlberto Escarpa
Feb 3, 2016·Analytical Chemistry·Benoit ChovelonCorinne Ravelet
Mar 5, 2016·Analytical and Bioanalytical Chemistry·Andreas WeltinGerald A Urban
Apr 1, 2016·Applied Microbiology and Biotechnology·Bernd Hofer

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Citations

Mar 15, 2018·The Analyst·Joonchul ShinHyo-Il Jung
Apr 4, 2021·International Journal of Molecular Sciences·Gheorghita MenghiuRaluca Ostafe

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Methods Mentioned

BETA
biosensors
biosensor
Assay

Software Mentioned

RLMP

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