Screening procedure for beta-adrenergic drugs in sports drug testing by immunological methods

Journal of Analytical Toxicology
R VenturaJordi Segura

Abstract

A systematic study of the cross-reactivities of beta-adrenergic agonists and antagonists and their metabolites with an ELISA test designed to detect terbutaline and other beta 2-agonists has been performed in order to evaluate the applicability of this immunological test as screening method in routine human sports drug testing. Concentration response curves were calculated. IC50 values from 1.7 to 21.5 ng/mL were obtained for beta 2-agonists, and values from 3.1 to 520.8 ng/mL were obtained for most of the beta-blockers. The highest cross-reactivity was mainly depending on the amino substituent of the molecules although the structure of the aromatic ring was also important. Minor changes in the aromatic ring, such as 4-hydroxylation for propranolol, did not largely influence the IC50 value. Distribution of control activities (percentage of optical density as compared with a blank sample) of urines obtained in controlled excretion studies (70 samples) and urines collected after competition in sports in which beta-blockers are recommended to be tested (147 samples) showed a very small overlapping between the subpopulations of positive and negative samples. Cutoff values of 45 or 60% control activity were proposed for routine anal...Continue Reading

Citations

Mar 13, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M RoigJ Segura
Oct 18, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lúcia DamascenoJordi Segura

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