Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester

Journal of Bioscience and Bioengineering
Ikuo KiraKenzo Yokozeki

Abstract

Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.

Citations

Jan 10, 2012·Annual Review of Food Science and Technology·Grant E DuBois, Indra Prakash

Related Concepts

Phenylalanine methyl ester, hydrochloride, (L)-isomer
Asparagine
Biotechnology
Catalysis
Citrobacter
Dipeptidases
SDS-PAGE
Paracolobactrum
Alkalescens-Dispar Group
Hydrogen-Ion Concentration

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