Scrotal heat stress causes a transient alteration in tight junctions and induction of TGF-β expression

International Journal of Andrology
Huan CaiY-X Liu

Abstract

Specialized junctions, which occur at sites of Sertoli-Sertoli and Sertoli-germ cell contact of seminiferous epithelium, play pivotal roles in spermatogenesis. Slight increase in scrotal temperature can induce oligospermia or azoospermia via increasing germ cell apoptosis. In this study, we demonstrated that the expression of tight junction (TJ) components, such as occludin, claudin-3 and zonula occludens-1 (ZO-1), was reduced 24-48h after a single mild scrotal heat exposure (43°C for 30min), whereas mRNA levels of claudin-11 were increased. Moreover, the protein localization of occludin and ZO-1 was lost from the blood-testis barrier (BTB) site, whereas claudin-11 immunostaining became diffuse and cytoplasmic 2days following heat exposure. Electron microscopic analysis showed that 2days after the heat treatment, the intercellular space between the two adjacent Sertoli cells was expanded, coupled with defragmentation of actin bundles and the endoplasmic reticulum. In addition, the TJ permeability increased significantly 2days after the heat exposure and recovered approximately 10days later. Heat-induced reversible BTB disruption was associated with a transient induction of transforming growth factor (TGF)-β2, -3 and p38 mitogen...Continue Reading

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Citations

Jul 22, 2014·The American Journal of Pathology·Masataka ChiharaYasuhiro Kon
Sep 26, 2012·Cellular and Molecular Life Sciences : CMLS·Byunghyuk KimKunsoo Rhee
Dec 17, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Su-Ren Chen, Yi-Xun Liu
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