SCT promoter methylation is a highly discriminative biomarker for lung and many other cancers

BioRxiv : the Preprint Server for Biology
Adwait SatheMichael Q Zhang

Abstract

Aberrant DNA methylation has long been implicated in cancers. In this work we present a highly discriminative DNA methylation biomarker for non-small cell lung cancers and fourteen other cancers. Based on 69 NSCLC cell lines and 257 cancer-free lung tissues we identified a CpG island in SCT gene promoter which was verified by qMSP experiment in 15 NSCLC cell lines and 3 immortalized human respiratory epithelium cells. In addition, we found that SCT promoter was methylated in 23 cancer cell lines involving >10 cancer types profiled by ENCODE. We found that SCT promoter is hyper-methylated in primary tumors from TCGA lung cancer cohort. Additionally, we found that SCT promoter is methylated at high frequencies in fifteen malignancies and is not methylated in ~1000 non-cancerous tissues across >30 organ types. Our study indicates that SCT promoter methylation is a highly discriminative biomarker for lung and many other cancers.

Related Concepts

Biological Markers
Malignant Neoplasms
Non-Small Cell Lung Carcinoma
Connective Tissue Cells
Epithelium
Lung
Methylation
Cell Line, Tumor
Promoter
Organ

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