Sculpting nanoparticle dynamics for single-bacteria-level screening and direct binding-efficiency measurement

Nature Communications
Y Z ShiA Q Liu

Abstract

Particle trapping and binding in optical potential wells provide a versatile platform for various biomedical applications. However, implementation systems to study multi-particle contact interactions in an optical lattice remain rare. By configuring an optofluidic lattice, we demonstrate the precise control of particle interactions and functions such as controlling aggregation and multi-hopping. The mean residence time of a single particle is found considerably reduced from 7 s, as predicted by Kramer's theory, to 0.6 s, owing to the mechanical interactions among aggregated particles. The optofluidic lattice also enables single-bacteria-level screening of biological binding agents such as antibodies through particle-enabled bacteria hopping. The binding efficiency of antibodies could be determined directly, selectively, quantitatively and efficiently. This work enriches the fundamental mechanisms of particle kinetics and offers new possibilities for probing and utilising unprecedented biomolecule interactions at single-bacteria level.

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Citations

Jan 10, 2019·Nature Reviews. Drug Discovery·Rachel S RileyMichael J Mitchell
Jul 28, 2020·Advanced Materials·Hongbao XinBaojun Li
Jan 30, 2020·Reports on Progress in Physics·Yansheng LiangMing Lei
Apr 28, 2020·Light, Science & Applications·Yuzhi ShiCheng-Wei Qiu
Aug 17, 2020·Physical Review Letters·Tongtong ZhuCheng-Wei Qiu
Oct 9, 2019·Advanced Science·Po-Hsun HuangTony Jun Huang
Apr 8, 2020·Light, Science & Applications·Aleksander BoguckiPiotr Kossacki
Dec 11, 2020·Micromachines·Shaobo LuoAi Qun Liu
Jan 31, 2021·Science Advances·Q SongP Genevet
Mar 28, 2021·Light, Science & Applications·Aleksander BoguckiPiotr Kossacki
Apr 1, 2021·Advanced Photonics Research·Yuzhi ShiAi Qun Liu
Apr 17, 2021·Light, Science & Applications·Yuzhi ShiCheng-Wei Qiu
May 28, 2021·Ultrasonics Sonochemistry·Yaolong Zhang, Xueye Chen
Dec 1, 2020·ACS Nano·Yuliang XieTony Jun Huang
Sep 11, 2021·ACS Sensors·Yuzhi ShiAi Qun Liu

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Methods Mentioned

BETA
protein folding
enzyme-linked
fluorescence-activated cell sorting
chip
optical
flow cytometry
irradiating
chips

Software Mentioned

Lumerical

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