Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Nak Yoon SungJae Youl Cho

Abstract

Flavonoids are plant pigments that have been demonstrated to exert various pharmacological effects including anti-cancer, anti-diabetic, anti-atherosclerotic, anti-bacterial, and anti-inflammatory activities. However, the molecular mechanisms in terms of exact target proteins of flavonoids are not fully elucidated yet. In this study, we aimed to evaluate the anti-inflammatory mechanism of scutellarein (SCT), a flavonoid isolated from Erigeron breviscapus, Clerodendrum phlomidis and Oroxylum indicum Vent that have been traditionally used to treat various inflammatory diseases in China and Brazil. For this purpose, a nitric oxide (NO) assay, polymerase chain reaction (PCR), nuclear fractionation, immunoblot analysis, a kinase assay, and an overexpression strategy were employed. Scutellarein significantly inhibited NO production in a dose-dependent manner and reduced the mRNA expression levels of inducible NO synthase (iNOS) and tumor necrosis factor (TNF)-α in lipopolysaccharide (LPS)-activated RAW264.7 cells. In addition, SCT also dampened nuclear factor (NF)-κB-driven expression of a luciferase reporter gene upon transfection of a TIR-domain-containing adapter-inducing interferon-β (TRIF) construct into Human embryonic kidney 2...Continue Reading

References

Nov 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·R M Clancy, S B Abramson
Oct 27, 1995·The Journal of Biological Chemistry·M OsuskyD Shalloway
Jan 14, 1999·The New England Journal of Medicine·R Ross
Nov 30, 2000·Arthritis Research·R W KinneG R Burmester
Sep 29, 2001·Nature Immunology·C Bogdan
Dec 9, 2004·International Immunology·Kiyoshi Takeda, Shizuo Akira
Aug 17, 2005·Current Drug Targets. Inflammation and Allergy·Nagatoshi Fujiwara, Kazuo Kobayashi
Mar 15, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shu Fang Liu, Asrar B Malik
Jul 18, 2008·Biochemical Society Transactions·John M O'Shea, Neil D Perkins
Dec 2, 2008·Journal of Ethnopharmacology·Yerra Koteswara RaoYew-Min Tzeng
Jun 17, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Muthu Kumaradoss Mohan Maruga Raja, Shri Hari Mishra
Jul 6, 2010·Biochemical Pharmacology·Kyung Mi LeeZigang Dong
Jan 5, 2011·Frontiers in Bioscience (Landmark Edition)·Yong Gyu LeeJae Youl Cho
Jul 26, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Sanjay BatraMalaya K Sahoo
May 4, 2012·Archives of Pharmacal Research·Jin-Ah LeeHyeun Kyoo Shin
Jul 28, 2012·International Immunopharmacology·Xiaojun ChenZhiliang Sun
Aug 28, 2012·Indian Journal of Pharmaceutical Sciences· HarminderA K Chaudhary
Jan 9, 2013·Journal of Ethnopharmacology·Deok JeongJae Youl Cho
Sep 7, 2013·PloS One·Jackeline Tiemy Guinoza SiraichiDiógenes Aparício Garcia Cortez
Nov 19, 2013·Biomolecules & Therapeutics·Cha Kyung YounYoung Jin Jeon
Jan 16, 2014·Pharmacology, Biochemistry, and Behavior·Hao TangJin-ao Duan
May 29, 2014·Journal of Ethnopharmacology·Eunji KimJae Youl Cho

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Citations

Apr 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunji KimJae Youl Cho
Jan 26, 2016·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Yong KimJae Youl Cho
Aug 24, 2017·Pharmaceutical Biology·Gyubyung ParkJae Youl Cho
Oct 30, 2019·BMC Complementary and Alternative Medicine·Jin-A KimGon Sup Kim
Jul 15, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sulgi YooJae Youl Cho
May 24, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Akash AhujaaJae Youl Cho
May 4, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young-Su YiJae Youl Cho
May 5, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Han Gyung KimJae Youl Cho
Oct 16, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Kon Kuk ShinJae Youl Cho
Aug 16, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·You Jin KimJae Youl Cho

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Methods Mentioned

BETA
transfection
Assay
profiler
PCR
nuclear translocation

Software Mentioned

SPSS

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