PMID: 8594023Nov 1, 1995Paper

SDH and actomyosin ATPase activities of different fiber types in rat diaphragm muscle

Journal of Applied Physiology
G C SieckJ F Watchko

Abstract

In the rat diaphragm muscle, the histochemical classification of type I, IIa, IIb, or IIx fibers was correlated with myosin heavy chain (MHC) immunoreactivity. Expression of MHC isoforms in single dissected fibers was also assessed electrophoretically. Most fibers (approximately 86%) expressed a single MHC isoform, and when present, coexpression of MHC-2X and MHC-2B isoforms was most prevalent. Type I and IIa fibers were the smallest, type IIb fibers were the largest, and type IIx fibers were intermediate. Succinate dehydrogenase (SDH) and calcium-activated myosin adenosinetriphosphatase (actomyosin ATPase) activities were measured with quantitative histochemical procedures. Type I and IIa fibers had the highest SDH activities, followed in rank order by type IIx and IIb fibers. Type I fibers had the lowest actomyosin ATPase activity, followed in rank order by type IIa, IIx, and IIb fibers. Across all fibers, there was an inverse relationship between fiber SDH activity and cross-sectional area and a positive correlation between fiber actomyosin ATPase activity and cross-sectional area. The SDH and actomyosin ATPase activities of muscle fibers were also inversely correlated. These phenotypic differences in SDH and ATPase activiti...Continue Reading

Citations

Feb 1, 1996·Journal of Neurocytology·Y S PrakashG C Sieck
Nov 1, 1996·Journal of Applied Physiology·M I LewisG C Sieck
Mar 28, 1998·American Journal of Respiratory and Critical Care Medicine·H RollierM Decramer
Aug 5, 2000·Journal of Applied Physiology·P C GeigerG C Sieck
Mar 16, 2002·Journal of Applied Physiology·Paige C GeigerGary C Sieck
Sep 16, 2005·Cell and Tissue Research·Luz M Acevedo, José-Luis L Rivero
Jan 12, 2008·Journal of Applied Physiology·Carlos B MantillaGary C Sieck
Dec 9, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stephan van der ZwaardRichard T Jaspers
Apr 8, 2020·BioMed Research International·Lulu DengChangchun Li
Oct 6, 2000·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·L T MalmgrenT Uno
Feb 22, 2001·Journal of Applied Physiology·G C Sieck, M Regnier
Aug 1, 1997·Muscle & Nerve·R H van BalkomC L van Herwaarden
May 10, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Vincent J CaiozzoKenneth M Baldwin
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Catherine CoiraultYves Lecarpentier
Jun 9, 2007·Journal of Applied Physiology·Gary C SieckY S Prakash
May 23, 2012·Journal of Strength and Conditioning Research·Nathan J HellyerCarlos B Mantilla
Feb 8, 2018·Physiology·Matthew J FogartyGary C Sieck
Nov 28, 2019·Journal of Applied Physiology·Matthew J FogartyGary C Sieck
Feb 6, 2020·Frontiers in Cell and Developmental Biology·Philippe Delmotte, Gary C Sieck
Feb 13, 2001·American Journal of Respiratory Cell and Molecular Biology·L A CallahanG Supinski
Oct 24, 1997·Journal of Applied Physiology·R H Van BalkomG C Sieck
Feb 4, 2003·Journal of Applied Physiology·Gary C SieckWen-Zhi Zhan
Apr 1, 1997·Journal of Applied Physiology·W Z ZhanG C Sieck
Jun 6, 1998·Journal of Applied Physiology·R H Van BalkomC L Van Herwaarden
Jan 14, 1999·Journal of Applied Physiology·G C SieckP N Dekhuijzen
Oct 17, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mohammed Z AllouhBenjamin W C Rosser
Oct 21, 2011·Physiological Reviews·Stefano Schiaffino, Carlo Reggiani
Dec 22, 1999·The American Journal of Physiology·C CoiraultY Lecarpentier
Mar 15, 2001·Journal of Applied Physiology·P C GeigerG C Sieck
Sep 16, 2003·Journal of Applied Physiology·Paul F Klawitter, Thomas L Clanton
Nov 1, 1996·Journal of Applied Physiology·G C SieckW Z Zhan
Feb 7, 2003·Journal of Applied Physiology·Carlos B Mantilla, Gary C Sieck
Jul 20, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Sarah M GreisingGary C Sieck

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