SDS-induced oligomerization of Lys49-phospholipase A2 from snake venom

Scientific Reports
Takashi MatsuiYoshikazu Tanaka

Abstract

Phospholipase A2 (PLA2) is one of the representative toxic components of snake venom. PLA2s are categorized into several subgroups according to the amino acid at position 49, which comprises either Asp49, Lys49, Arg49 or Ser49. Previous studies suggested that the Lys49-PLA2 assembles into an extremely stable dimer. Although the behavior on Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing or non-reducing conditions suggested the presence of intermolecular disulfide bonds, these bonds were not observed in the crystal structure of Lys49-PLA2. The reason for this discrepancy between the crystal structure and SDS-PAGE of Lys49-PLA2 remains unknown. In this study, we analyzed a Lys49-PLA2 homologue from Protobothrops flavoviridis (PflLys49-PLA2 BPII), by biophysical analyses including X-ray crystallography, SDS-PAGE, native-mass spectrometry, and analytical ultracentrifugation. The results demonstrated that PflLys49-PLA2 BPII spontaneously oligomerized in the presence of SDS, which is one of the strongest protein denaturants.

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Citations

Apr 9, 2020·Biochemical Society Transactions·Jia Jin Hiu, Michelle Khai Khun Yap
Mar 17, 2021·Biochimica Et Biophysica Acta. Proteins and Proteomics·Débora Andrade-SilvaSolange M T Serrano
Mar 31, 2021·Toxicon : Official Journal of the International Society on Toxinology·Norival Alves Santos-FilhoEduardo Maffud Cilli
Dec 29, 2020·Biophysical Chemistry·Virjinia DoltchinkovaPlamena R Angelova

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Methods Mentioned

BETA
X-ray
electrophoresis

Software Mentioned

MolProbity
SEDNTERP
Phaser
MassLynx
SEDPHAT
SEDFIT
XDS
phenix

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