PMID: 699039Sep 1, 1978Paper

Sea urchin nuclei use RNA polymerase II to transcribe discrete histone RNAs larger than messengers

Cell
S LevyL H Kedes

Abstract

RNA transcribed in isolated sea urchin nuclei and assayed by hybridization to histone genes cloned in E. coli contains sequences homologous to each of the five histone genes. Histone RNA is synthesized exclusively from the same DNA strand which is the template in vivo. Synthesis of the histone gene transcripts is sensitive to alpha-amanitin concentrations which inhibit RNA polymerase II activity. The fraction of histone RNA synthesized in vitro is comparable at two developmental stages to the fraction synthesized in vivo. The nuclear histone transcripts contain sequences homologous to spacer DNA regions present between the coding regions of the 6500 base pair (bp) histone gene repeat unit. The transcription of spacer sequences was demonstrated by hybridization of the nuclear transcripts to subcloned spacer DNA. Although the bulk of the RNA transcripts are greater than 2000 bases long, the histone-specific transcripts are of discrete sizes ranging from 100 bases to about 1100 bases long. Each histone gene hybridizes with at least one of the larger transcripts and with a different subset of smaller RNAs. We do not detect any giant polycistronic transcript spanning the entire histone repeat unit.

References

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Citations

Nov 1, 1983·Developmental Biology·D V DeLeonR C Angerer
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R Grosschedl, M L Birnstiel
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H G Stunnenberg, M L Birnstiel
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J A Knowles, G J Childs
Jul 11, 1979·Nucleic Acids Research·M BusslingerM L Birnsteil
May 15, 1980·Journal of Molecular Biology·G SpinelliM Ciaccio
May 1, 1981·European Journal of Biochemistry·M Buongiorno-NardelliE Di Mauro
May 31, 1979·Molecular Biology Reports·K ScherrerA Therwath
Mar 1, 1982·CRC Critical Reviews in Biochemistry·Y V Ilyin, G P Georgiev
Aug 1, 1981·Cell·C C Hentschel, M L Birnstiel
Dec 15, 1979·Journal of Molecular Biology·E ProbstM L Birnstiel

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