Seabream ghrelin: cDNA cloning, genomic organization and promoter studies

The Journal of Endocrinology
Chung-Man YeungChristopher H K Cheng

Abstract

Recent studies have indicated that ghrelin stimulates growth hormone release from the pituitary via the growth hormone secretagogue receptor (GHSR). We have previously isolated two GHSR subtypes from the pituitary of the black seabream Acanthopagrus schlegeli. In the present study, we have cloned and characterized ghrelin from the same fish species at both the cDNA and gene levels. The full-length seabream ghrelin cDNA, isolated from sea-bream stomach using a novel approach by exploiting a single conserved region in the coding region, was found to encode a prepropeptide of 107 amino acids, with the predicted mature ghrelin peptide consisting of 20 amino acids (GSSFLSPSQKPQNRGKSSRV). Embedded in this full-length cDNA is a putative fish orthologue of the recently reported mammalian obestatin peptide. The ghrelin gene in black seabream, obtained by genomic PCR, was found to encompass four exons and three introns, possessing the same structural organization as in tilapia and goldfish, but different from that in rainbow trout. In addition, a 2230-bp 5'-flanking region of the seabream ghrelin gene was obtained by genome walking. Sequence analysis revealed that, as in the case of the human ghrelin gene, there is neither a GC box nor a...Continue Reading

Citations

May 10, 2012·General and Comparative Endocrinology·Atsushi SudaHironori Ando
Feb 9, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Shelly A CruzPung Pung Hwang
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Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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