Seamless stitching of tile scan microscope images

Journal of Microscopy
F B LegesseR Heintzmann

Abstract

For diagnostic purposes, optical imaging techniques need to obtain high-resolution images of extended biological specimens in reasonable time. The field of view of an objective lens, however, is often smaller than the sample size. To image the whole sample, laser scanning microscopes acquire tile scans that are stitched into larger mosaics. The appearance of such image mosaics is affected by visible edge artefacts that arise from various optical aberrations which manifest in grey level jumps across tile boundaries. In this contribution, a technique for stitching tiles into a seamless mosaic is presented. The stitching algorithm operates by equilibrating neighbouring edges and forcing the brightness at corners to a common value. The corrected image mosaics appear to be free from stitching artefacts and are, therefore, suited for further image analysis procedures. The contribution presents a novel method to seamlessly stitch tiles captured by a laser scanning microscope into a large mosaic. The motivation for the work is the failure of currently existing methods for stitching nonlinear, multimodal images captured by our microscopic setups. Our method eliminates the visible edge artefacts that appear between neighbouring tiles by ...Continue Reading

References

Nov 30, 2006·Microscopy Research and Technique·Philippe Thévenaz, Michael Unser
Apr 7, 2009·Bioinformatics·Stephan PreibischPavel Tomancak
Aug 8, 2012·The Journal of Cell Biology·Frank G A FaasRaimond B G Ravelli
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri

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Citations

Jan 31, 2019·Lab on a Chip·Sara KheireddineSebastian Wachsmann-Hogiu
Oct 3, 2018·Optics Letters·Sandro HeukeHervé Rigneault
Nov 4, 2016·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Fisseha Bekele LegesseJürgen Popp
Oct 24, 2019··Thomas Bocklitz, Thomas Bocklitz

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