Search for Viral Infections in Cerebrospinal Fluid From Patients With Autoimmune Encephalitis

Open Forum Infectious Diseases
Karol PerlejewskiTomasz Laskus

Abstract

It has been reported that virus-mediated brain tissue damage can lead to autoimmune encephalitis (AE) characterized by the presence of antibodies against neuronal surface antigens. In the study, we investigate the presence of viruses in cerebrospinal fluid (CSF) from patients with AE using reverse transcription polymerase chain reaction (RT-PCR)/PCR and shotgun metagenomics. CSF samples collected from 200 patients with encephalitis were tested for the presence of antibodies against antiglutamate receptor (NMDAR), contactin-associated protein 2 (CASPR2), glutamate receptors (type AMPA1/2), leucine-rich glioma-inactivated protein 1 (LGI1), dipeptidyl aminopeptidase-like protein 6 (DPPX), and GABA B receptor, and those found positive were further analyzed with real-time RT-PCR/PCR for common viral neuroinfections and shotgun DNA- and RNA-based metagenomics. Autoantibodies against neuronal cells were detected in CSF from 8 individuals (4% of all encephalitis patients): 7 (3.5%) had anti-NMDAR and 1 (0.5%) had anti-GABA B. RT-PCR/PCR identified human herpes virus type 1 (HSV-1; 300 copies/mL) and the representative of Enterovirus genus (550 copies/mL) in 1 patient each. Torque teno virus (TTV) was found in another patient using meta...Continue Reading

References

Nov 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C A GlaserJ H Tureen
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Oct 19, 2010·The Lancet Infectious Diseases·Julia GranerodUNKNOWN UK Health Protection Agency (HPA) Aetiology of Encephalitis Study Group
Oct 29, 2010·Genome Research·Gerton Lunter, Martin Goodson
Jan 28, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Mary S GableCarol A Glaser
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jan 3, 2013·Annals of Neurology·Harald PrüssKlaus-Peter Wandinger
Jul 19, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A VenkatesanUNKNOWN International Encephalitis Consortium
Oct 22, 2013·BMC Neurology·Abhinbhen SarayaThiravat Hemachudha
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 6, 2014·Current Opinion in Neurology·Thaís ArmangueJosep Dalmau
Jun 12, 2014·Annals of Neurology·Emilia H MoscatoRita J Balice-Gordon
Nov 8, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Sylwia RynansGrażyna Młynarczyk
Nov 14, 2014·Brain : a Journal of Neurology·Jesús PlanagumàJosep Dalmau
Oct 23, 2015·Neurology·Thaís ArmangueUNKNOWN Spanish Prospective Multicentric Study of Autoimmunity in Herpes Simplex Encephalitis
Jan 13, 2016·Journal of Clinical Neurology·Eric Lancaster
Feb 26, 2016·Lancet Neurology·Francesc GrausJosep Dalmau
Jul 1, 2013·European Neurological Review·Frank LeypoldtJosep Dalmau
Jan 20, 2017·Journal of Epilepsy Research·Sang Kun Lee, Soon-Tae Lee
Feb 9, 2017·Archives of Pathology & Laboratory Medicine·Robert SchlabergUNKNOWN Microbiology Resource Committee of the College of American Pathologists
Jan 25, 2018·Arquivos de neuro-psiquiatria·Lívia Almeida DutraRomana Hoftberger
Feb 6, 2018·Therapeutic Advances in Neurological Disorders·Yong-Won ShinKon Chu
Mar 25, 2018·Annals of Neurology·Stefanie H MuellerUNKNOWN German Network for Research on Autoimmune Encephalitis (GENERATE)
Mar 29, 2018·Epilepsia Open·Anjan NibberBethan Lang
Apr 10, 2018·Neurology. Neuroimmunology and Neuroinflammation·Amy SalovinJessica A Panzer
Jun 16, 2018·Neurology. Neuroimmunology and Neuroinflammation·Russell C Dale, Margherita Nosadini
Jul 14, 2018·Journal of Neurology·Chloé BostJerome Honnorat
Oct 27, 2018·Frontiers in Neurology·Gerda RickenRomana Höftberger
Jan 31, 2019·Neurology. Neuroimmunology and Neuroinflammation·Preeti A PrakashJoseph S Kass
Aug 14, 2019·Frontiers in Neurology·Tetyana Blinder, Jan Lewerenz
Aug 28, 2019·Clinical Immunology : the Official Journal of the Clinical Immunology Society·P KaragianniH M Moutsopoulos

❮ Previous
Next ❯

Datasets Mentioned

BETA
AB017610.1

Methods Mentioned

BETA
PCRs
RNA-Seq
PCR

Software Mentioned

CLC Genomics Workbench
FastQC
phyloseq package
Stampy
Trimmomatic
R
Bowtie2
SAMtools

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.