Searching cell-secreted proteomes for potential urinary bladder tumor markers

Proteomics
Chiao-Yun LinBenjamin Yat-Ming Yung

Abstract

To search for biomarkers critical for bladder carcinoma diagnosis and prognosis, secreted proteomes of highly malignant U1 and pre-malignant U4 cell lines were initially analyzed. Proteins in the culture media of the U1 and U4 cell lines were systematically examined by SDS-PAGE combined with MALDI-TOF MS. Among them, expression of pro-u-plasminogen activator (pro-u-PA) was confirmed by Western blot analysis and further evaluated. In analyzing urine samples from bladder cancer patients and normal subjects, we established a statistically significant relationship between the low level and absence of pro-u-PA in urine with high stages and grades of the tumor samples. Constitutive expression of Ras dominant negative protein led to increased expression of pro-u-PA in culture media, indicating that the loss of pro-u-PA is associated with oncogenic transformation. Analysis of cancer-secreted proteomes can be a feasible, non-invasive and efficient strategy for searching potential bladder tumor biomarkers. Our work also has identified the loss of pro-u-PA in urine as potential marker of more advanced bladder carcinoma.

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Citations

Dec 19, 2009·Molecular & Cellular Proteomics : MCP·Rajaventhan SrirajaskanthanNicholas J Beaumont
Feb 27, 2010·BMC Cancer·Susanne Klein-ScoryIrmgard Schwarte-Waldhoff
Sep 18, 2008·Journal of Translational Medicine·Hua XueMaode Lai
Feb 28, 2007·Pharmacogenomics·Glen L Hortin, Denis Sviridov
Apr 12, 2007·Expert Review of Proteomics·Yetrib Hathout
Jul 20, 2010·Journal of Proteomics·Manousos Makridakis, Antonia Vlahou
Nov 17, 2009·The Journal of Urology·Ke-Hung TsuiBenjamin Yat-Ming Yung
Jun 21, 2011·Journal of Biomedicine & Biotechnology·Chibo Liu
Oct 19, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Zoraida AndreuMaría Yáñez-Mó
Feb 28, 2013·Nature Reviews. Urology·Steven L WoodRosamonde E Banks
Jul 12, 2017·Expert Review of Proteomics·Yung-Chin HsiaoJau-Song Yu
Feb 6, 2017·Bladder Cancer·Maria Frantzi, Antonia Vlahou
Apr 29, 2010·Journal of Proteome Research·Manousos MakridakisAntonia Vlahou

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