Abstract
Differential proteomic analysis has been performed on the cerebrospinal fluid (CSF) of six healthy and six patients suffering form sporadic Creutzfeldt-Jakob disease (sCJD), age- and sex-matched, after immuno-subtraction of albumin and immunoglobulins. These maps have revealed 28 polypeptide chains differentially modulated in the sCJD samples, of which 10 appeared to be up-regulated, the remaining 18 being down-regulated. Among those, 13 could be identified upon digestion and MALDI-TOF, MS analysis. In addition, the strong modulation of cystatin C was also confirmed by immunoblot analysis and the highly altered level of the 14-3-3 proteins that escaped detection by 2-D mapping, could be assessed by Western blots and immuno-detection of monomeric and homo- and hetero-dimeric 14-3-3 isotypes. In search for a panel of potential markers for sCJD, we highlight cystatin C, 14-3-3 proteins, transferrin, ubiquitin, Apoliprotein J and perhaps some of the still unidentified, but strongly modulated polypeptide chains detected in the differential map.
References
May 1, 1982·Journal of Neurochemistry·P F BostonR J Thompson
Sep 26, 1996·The New England Journal of Medicine·G HsichM G Harrington
Jan 1, 1997·International Psychogeriatrics·K Iqbal, I Grundke-Iqbal
Mar 11, 1999·Annals of Neurology·S KiuruM Haltia
May 18, 1999·Biochemical and Biophysical Research Communications·V P ChauhanH M Wisniewski
Jan 21, 2000·Brain Research·I RayV P Chauhan
Feb 16, 2000·Journal of Neuroimmunology·F LicastroL M Grimaldi
Apr 19, 2000·Acta Neurologica Scandinavica·J KálmánZ Janka
Aug 11, 2000·Microscopy Research and Technique·M CaleroJ Ghiso
Jan 3, 2001·Neurology·A NagaiS Kobayashi
Mar 22, 2002·Acta Neuropathologica·Kensuke SasakiToru Iwaki
Apr 18, 2002·Neurobiology of Aging·Xiaoyan WangM Ilyas Kamboh
Apr 26, 2002·Neuroreport·Pia DavidssonKaj Blennow
Jan 2, 2003·Annals of Neurology·Steven T DeKoskyM Ilyas Kamboh
Feb 19, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Atsushi NagaiShotai Kobayashi
Mar 28, 2003·Proteins·Hadar BenyaminiRuth Nussinov
Aug 19, 2003·Proteomics·Odile CarretteJean-Charles Sanchez
Sep 3, 2003·Nature Reviews. Neuroscience·Daniela BergOlaf Riess
Oct 16, 2003·Brain Research. Molecular Brain Research·Maja PuchadesPia Davidsson
Nov 5, 2003·Proteomics·Han-Ling YuHyman M Schipper
Mar 5, 2004·Journal of the National Cancer Institute·Eleftherios P Diamandis
Jun 8, 2004·Molecular Neurobiology·Jing Zhang, David R Goodlett
Jun 30, 2004·Free Radical Biology & Medicine·Silvia Mandel, Moussa B H Youdim
Jul 6, 2004·Acta Neuropathologica·M FreixesI Ferrer
Jul 13, 2004·The Journal of Biological Chemistry·Gianluigi ZanussoSalvatore Monaco
Jul 24, 2004·Nature Medicine·Ella Bossy-WetzelStuart A Lipton
Jul 27, 2004·Proteomics·Jean-Charles SanchezDenis F Hochstrasser
Sep 21, 2004·Proteomics·Annalisa CastagnaPier Giorgio Righetti
Jan 25, 2005·Disease Markers·Walter S LiggettLisa H Cazares
Jan 26, 2005·Proteomics·Emilio MarengoPier Giorgio Righetti
Mar 8, 2005·Proteomics·Chiara PiubelliEnrico Domenici
May 11, 2005·Neurology·G ZanussoS Monaco
Citations
Aug 10, 2006·Acta Neuropathologica·Yuji MizunoKoichi Okamoto
May 14, 2008·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A KulakowskaP A Janmey
Sep 23, 2006·BMC Neurology·Constanze Boesenberg-GrosseInga Zerr
Jul 2, 2009·Genome Medicine·Maurizio PocchiariFranco Cardone
Dec 6, 2008·PloS One·Gino MieleAdriano Aguzzi
Mar 17, 2011·PloS One·Ajay SinghNeena Singh
Dec 17, 2011·International Journal of Molecular Sciences·Alberto GajofattoSalvatore Monaco
Aug 6, 2013·Molecular Neurobiology·Cao ChenXiao-Ping Dong
Jul 26, 2011·Neurochemical Research·Xi PengXuefeng Wang
Jul 20, 2012·Expert Review of Proteomics·Di Ma, Lingjun Li
Dec 15, 2010·Expert Review of Proteomics·Antonio QualtieriAldo Quattrone
Dec 8, 2010·Proteomics. Clinical Applications·Carlos E RodríguezEncarnació Riudor
Jul 6, 2010·Journal of Immunological Methods·Annaby Moussa NdjoleAnders Larsson
Sep 25, 2008·Proteomics·Peter BrechlinMarkus Otto
Aug 1, 2007·Proteomics. Clinical Applications·Frédérique LisacekRon D Appel
Sep 1, 2007·Proteomics. Clinical Applications·Michele FioriniSalvatore Monaco
Feb 20, 2007·Proteomics·Murat EravciAndreas Baumgartner
Nov 3, 2009·Proteomics·Petra SteinackerMarkus Otto
Oct 5, 2010·International Journal of Alzheimer's Disease·Philipp SpitzerJens Wiltfang
Mar 13, 2010·Journal of Biomedicine & Biotechnology·Rhiannon L C H HuzarewichStephanie A Booth
Dec 14, 2007·Molecular BioSystems·Mark R WilsonStephen Poon
Oct 1, 2009·Physiological Reviews·Adriano Aguzzi, Anna Maria Calella
Mar 22, 2015·Expert Review of Proteomics·Roger A MooreSuzette A Priola
Jan 1, 2015·Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·I Zerr, T A Polyakova
Sep 28, 2010·Journal of Proteome Research·Joanna GawineckaInga Zerr