Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers

Oncogene
E ForgacsJ D Minna

Abstract

RepX represents a new informatics approach to probe the UniGene database for potentially polymorphic repeat sequences in the open reading frame (ORF) of genes, 56% of which were found to be actually polymorphic. We now have performed mutational analysis of 17 such sites in genes not found to be polymorphic (<0.03 frequency) in a large panel of human cancer genomic DNAs derived from 31 lung, 21 breast, seven ovarian, 21 (13 microsatellite instability (MSI)+ and eight MSI-) colorectal cancer cell lines. In the lung, breast and ovarian tumor DNAs we found no mutations (<0.03-0.04 rate of tumor associated open reading frame mutations) in these sequences. By contrast, 18 MSI+ colorectal cancers (13 cancer cell lines and five primary tumors) with mismatch repair defects exhibited six mutations in three of the 17 genes (SREBP-2, TAN-1, GR6) (P<0.000003 compared to all other cancers tested). We conclude that coding region microsatellite alterations are rare in lung, breast, ovarian carcinomas and MSI (-) colorectal cancers, but are relatively frequent in MSI (+) colorectal cancers with mismatch repair deficits.

References

Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X HuaX Wang
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·K OrthJ Sambrook
Feb 9, 1996·The Journal of Biological Chemistry·R D Wells
Aug 8, 1996·Nature·S MalkhosyanM Perucho
Nov 5, 1997·Biochemical and Biophysical Research Communications·K MatuokaT Takenawa
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·J W FondonH R Garner
Mar 26, 1999·American Journal of Human Genetics·D L MarescoJ Boyd
Aug 7, 1999·The Journal of Biological Chemistry·J P Jakupciak, R D Wells
Mar 25, 2000·Endocrine-related Cancer·S KrajewskiJ C Reed
Jul 13, 2000·American Journal of Human Genetics·J D WrenH R Garner

❮ Previous
Next ❯

Citations

Nov 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Jose RussoIrma H Russo
Jan 2, 2008·Cell Research·Irina V Kovtun, Cynthia T McMurray
Jan 11, 2007·American Journal of Clinical Pathology·Sanja DacicSamuel A Yousem
Feb 15, 2011·Genes, Chromosomes & Cancer·Cristi L GalindoHarold R Garner
Jun 30, 2004·Trends in Endocrinology and Metabolism : TEM·Jose Russo, Irma H Russo
Nov 1, 2016·Nature Medicine·Ronald J HauseStephen J Salipante
Jan 6, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mihai D NiculescuSteven H Zeisel
Sep 8, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Tatsuhiro ShibataSetsuo Hirohashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.

Related Papers

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Fabien K BakshSamuel A Yousem
Journal of Cellular Biochemistry
C M Whitehead, J L Salisbury
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
L T HansenL Nørgård Petersen
© 2021 Meta ULC. All rights reserved