Searching for new markers of endogenous steroid administration in athletes: "looking outside the metabolic box"

Forensic Science International : Synergy
Adam T CawleyRymantas Kazlauskas

Abstract

A simple means of detecting the abuse of steroids that also occur naturally is a problem facing doping control laboratories. Specific markers are required to allow the detection of the administration of these steroids. These markers are commonly measured using a set of data obtained from the screening of samples by gas chromatography-mass spectrometry (GC-MS). Doping control laboratories further need to confirm identified abuse using techniques such as gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). An interesting urinary species was found while following the pharmacokinetics and changes to the steroid profile from single and multiple oral doses of the International Olympic Committee/World Anti Doping Agency (IOC/WADA) prohibited substance, dehydroepiandrosterone (DHEA). The urine samples collected from the administration studies were subject to GC-MS and GC-C-IRMS steroid analysis following cleanup by solid phase extraction techniques. A useful urinary product of DHEA administration was detected in the urine samples from each of the administration studies and was identified by GC-MS experiments to be 3alpha,5-cyclo-5alpha-androstan-6beta-ol-17-one (3alpha,5-cyclo). This compound occurs naturally but ...Continue Reading

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Citations

Aug 17, 2010·Analytical and Bioanalytical Chemistry·Oscar J PozoJordi Segura
Mar 31, 2011·Analytical and Bioanalytical Chemistry·Thomas PiperMartial Saugy
May 28, 2005·Forensic Science International : Synergy·Sarah BensonClaude Roux
Jul 20, 2010·Annual Review of Analytical Chemistry·Larry D Bowers
Jul 20, 2010·Annual Review of Analytical Chemistry·Suzanne Bell
Nov 17, 2009·Analytical and Bioanalytical Chemistry·Ilse BecueCarlos Van Peteghem
Aug 25, 2011·Forensic Science International : Synergy·Christian Reichel
Aug 13, 2011·Forensic Science International : Synergy·Julien BoccardSerge Rudaz
Dec 24, 2010·Forensic Science International : Synergy·Adam T CawleyAdrian V George
May 12, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Rodrigo AguileraFrancisco Aquino Neto
Jan 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christophe SaudanMartial Saugy
Nov 18, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·P Van RenterghemF T Delbeke
Feb 20, 2008·Forensic Science International : Synergy·Christophe SaudanMartial Saugy
Jun 5, 2008·Journal of Mass Spectrometry : JMS·Adam T Cawley, Ulrich Flenker
Nov 26, 2008·Rapid Communications in Mass Spectrometry : RCM·Adam T CawleyAdrian V George
Jun 24, 2008·Journal of Mass Spectrometry : JMS·Ute MareckWilhelm Schänzer
Jan 5, 2011·Drug Testing and Analysis·Thomas PiperWilhelm Schänzer
Jan 5, 2011·Drug Testing and Analysis·Pieter Van RenterghemFrans Delbeke
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Oct 14, 2010·Rapid Communications in Mass Spectrometry : RCM·Thomas PiperWilhelm Schänzer
Dec 29, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Maria Kristina Parr, Wilhelm Schänzer
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Nov 9, 2019·Drug Testing and Analysis·Dayamin Martinez-BritoFrancesco Botrè
Jul 22, 2009·Analytical Chemistry·Jeroen C W RijkMichel W F Nielen

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