Abstract
Previous studies have suggested that peripheral blood T cell cytokine release may reflect the situation in the bronchial mucosa in atopic asthmatics. We have therefore examined spontaneous, rye grass pollen-stimulated, and PHA-stimulated secretion of IL-2, IL-4, IL-5, and IFN-gamma in cultures of peripheral blood mononuclear cells (PBMC) from atopic asthmatic, atopic non-asthmatic and normal controls in and out of the rye grass pollen season. Compared to normal controls, both atopic groups showed higher IL-2 and IL-5 production (p < 0.01 and p < 0.05) and higher percentages of CD4 cells expressing CD25 and HLA-DR (p < 0.05) in response to rye grass pollen, elevated IL-4 production in response to PHA (p < 0.01), and a non-significant trend towards lower IFN-gamma production. However, the capacity of PBMC from atopic asthmatics to produce IL-5 was greater than that for atopic non-asthmatics (p < 0.05). Furthermore, seasonal changes in symptom score, FEV1 % predicted, and PD20 methacholine correlated with seasonal elevated production of IL-5 by PBMC in the asthmatic group (r = 0.862, -0.679, and -0.620; p < 0.01, 0.02, and 0.05, respectively). Our results suggest that elevated production of IL-2 and IL-4 and higher percentages of ...Continue Reading
Citations
May 20, 2003·Veterinary Immunology and Immunopathology·Shunsuke FujiwaraHajime Tsujimoto
Sep 9, 2000·Immunology Letters·R MovérareG Stålenheim
Sep 12, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·N J KenyonN N Jarjour
Dec 16, 2000·Allergy·J BettiolM Malaise
Sep 14, 2001·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·J M Smart, A S Kemp
Feb 26, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R M O'BrienW R Thomas
Mar 26, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Y I KohH C Lee
Aug 10, 2000·Scandinavian Journal of Immunology·R MovérareG Stâlenheim
Nov 20, 1998·Allergy·C AbbalJ Pène
Apr 8, 1999·Thorax·J TemelkovskiR K Kumar
May 1, 1998·American Journal of Respiratory and Critical Care Medicine·C TangE H Walters
Mar 1, 1997·Respirology : Official Journal of the Asian Pacific Society of Respirology·C TangE H Walters
Jun 28, 2001·Clinical and Diagnostic Laboratory Immunology·C CamillaV Fert
Oct 20, 2001·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·M MajoriA Pesci
Jun 1, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·P S FosterR K Kumar
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Wayne R ThomasWendy-Anne Smith